The unique design of the EPIK™ miRNA Assays confer distinct advantages over other approaches to miRNA detection. A stem-loop structure-based assays are designed using proprietary thermodynamics-based algorithms, enabling EPIK™ miRNA Assays to discrimination between miRNA sequences with high similarity and SensiSMART™ qPCR technology leads to remarkable sensitivities as well as extremely low background, enabling the accurate detection of very low miRNA levels.
Using advanced design concepts, a proprietary algorithm has been developed by MiRXES™ to create modified stem-loop miRNA specific reverse transcription primers and hemi-nested real-time PCR primer combinations, to maximize miRNA detection sensitivity while minimizing non-specific interactions. The assays use SYBR® Green rather than a probe-based system for detection, allowing rapid amplification when required (Fig. 1). The resulting real-time PCR assays use SensiSMART™ enabling detection of extremely low levels of miRNA with high specificity, allowing the discrimination between closely related miRNA sequences (Fig. 2).
Performance of these assays have been validated to detect as few as 100 copies of template per RT reaction (Fig. 3) with excellent assay efficiency and linearity
The EPIK miRNA Hi-ROX Kit is designed to allow you to do 4 x 100 reactions from a choice of over 27,000 miRNA primer sets. You can choose from 1 up to 4 different miRNA primer sets.
ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, OneStep™, OneStep™ Plus, as well as other instruments that do not require the use of a passive reference.
|20x RT Primers||4 x 16 µL|
|10x PCR Primers||4 x 200 µL|
|EPIK RT Buffer||1 x 160 µL|
|EPIK RT Enzyme||1 x 40 µL|
|2x SensiSMART™ Hi-ROX Master Mix||4 x 1 mL|
|DEPC Water||5 x 1.8 mL|
All components are shipped on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.
On Dry Ice or Blue Ice.