• Ordering

    Cat. No.
    Size
    BIO-86105
    500 Reactions
    BIO-86120
    2000 Reactions

    *link will take you to our distributor which supports all Academic and Government Institutes. All other customers please submit your order to orders@meridianlifescience.com

    Description

    A highly inhibitor-resistant qPCR master mix that provides quick and easy extraction and amplification of DNA from a variety of tissue types. The supermix maximizes sensitivity while simultaneously minimizing the effect of blood, tissue and plant PCR inhibitors, to deliver greater experiment success rates.

    Product Highlights

    • Robust: optimized proprietary buffer system designed for overcoming common PCR inhibitors in crude lysate or unprocessed blood, tissue and plant samples
    • Specific: antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
    • Sensitive: reliable quantification of low abundance targets and scarce samples
    • Reproducible: consistent results between technical replicates for increased confidence in results
    • Fast: delivers reproducible, multiplex accurate assay results in as little as 30 minutes

    Product Description

    SensiFAST™ Direct Probe SuperMix is a combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an antibody-mediated hot-start polymerase, dNTPs and MgCl2. It has been designed for highly reproducible, accurate assay results in the presence of inhibitors, making it ideal for direct amplification directly from the most challenging samples.

    The advanced buffer chemistry and enhancers has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe‑detection technology, including TaqMan®, Scorpions® and molecular beacon probes, making SensiFAST Direct Probe SuperMix perfect for multiplexing, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.

    Applications

    • Gene expression analysis
    • Viral and bacterial detection
    • GMO testing
    • SNP genotyping
    • Mutation detection
    • Environmental monitoring
    Main
    Inhibitor-resistant qPCR master mix for greater experiment success rates.

    Instrument Compatibility

    SensiFAST Direct Probe SuperMix is a No-ROX mix, please refer to the Real-Time PCR Selection Chart to confirm the SensiFAST Direct Probe (No-ROX) SuperMix is compatible with your instrument.


    Resources

    Certification of Analysis (COAs)

    Specification

    Components

     Product Name

     500 x 20 µL Reactions

     2000 x 20 µL Reactions

     SensiFAST Probe Direct Supermix (2x)

     5 x 1 mL

     4 x 5 mL

    Volume

    BIO-86105: 500 x 20 µL Reactions: 5 x 1 mL
    BIO-86120: 2000 x 20 µL Reactions: 4 x 5 mL

    Storage & Stability

    All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.

    Shipping conditions

    On Dry Ice or Blue Ice.



    .