- Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets
- Reproducible – consistent results between technical replicates for increased confidence in results
- Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
- Robust – reliable detection of RNA targets from a broad range of sample types
- Fast – delivers reproducible, accurate assay results in as little as 40 minutes
The SensiFAST™ SYBR® No-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube.
An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.
The SensiFAST SYBR® No-ROX One-Step Kit has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.
- Gene expression analysis
- Pathogen detection
- RNA viral pathogen detection
- Genetic profiling
- miRNA profiling / quantification
- Genetically modified organisms (GMO) characterization
After getting nowhere with other kits we tried the SensiFAST SYBR One-Step Kit and saw results first time, I cannot describe how wonderful this made us feel.
Asia-Pacific Special Nutrients Sdn. Bhd.
Instrument CompatibilityPlease refer to the Real-Time PCR Selection Chart to confirm the SensiFAST SYBR® No-ROX One-Step Kit is compatible with your instrument.
Certification of Analysis (COAs)
100 x 20 µL Reactions
500 x 20 µL Reactions
SensiFAST SYBR® No-ROX One-Step Mix (2x)
1 x 1 mL
5 x 1 mL
RiboSafe RNase Inhibitor
1 x 40 µL
1 x 200 µL
1 x 20 µL
1 x 100 µL
1 x 1.8 mL
2 x 1.8 mL
- BIO-72001: 100 x 20 µL Reactions: 1 x 1 mL
- BIO-72005: 500 x 20 µL Reactions: 5 x 1 mL
Storage & Stability
All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.
Human - Rosato R.R., et al. Cancer Res. 67: 9490-500 (2007)
Rat - Remund K., et al. Expt. Lung Res. 35: 359-370 (2009)
Drosophila - Brown A.E., et al. PloS ONE 4: e4490 (2009)
Nitrogen-fixing bacteria - Bahlawane C., et al. Mol. Plant-Microbe Inter. 21: 1498-1509 (2008)
Diphtheria bacteria - Jochmann N., et al. Microbiology 155: 1459-1477 (2009)
Green algae - Wobbe L., et al. PNAS 106: 13290-13295 (2009)
Bovine virus - Park S-I., et al. J. Virol. Methods. 159: 64-6 (2009)
For cDNA/DNA templates SensiFAST SYBR® and Probe kits are used. To give you some idea of a few of the types of templates used with these kits:
Stem Cell - Bernardo A.S., et al. Stem Cells 27(2): 341 -351 (2008)
Cow - Baumert A., et al. J. Dairy Research 76(3): 356-364 (2009)
Mouse - Hoyles R.K., et al. Arthritis Rheum. 58(4): 1175-88 (2008)
Quail - De Winter P., et al. British Poultry Science 49(5): 566 – 573 (2008)
Insect - Bass C., et al. Malaria journal 6 111 (2007)
Fish - Miller M.R., et al. J. Nutr. 138(11): 2179-2185 (2008)
Crab - Wilcockson D.C. & Webster S.G. Gen. & Comp. Endo. 156: 113–125 (2008)
Nematode - Murray S.L., et al. Mol. Plant-Microbe Interactions 20(11): 1431–1438 (2007)
Plant - Hecht V., et al. Plant Physiology Preview DOI:10.1104/pp.107.096818 (2007)
Yeast - Kawauchi J., et al. Genes & Dev. 22: 1082-1092 (2008)
E.coli Bacteria - Saeed H.A., et al. Research J. Microbiology 4(4); 173-177 (2009)
Virus - Muscillo M., et al. Water, Air, & Soil Pollution 191: 1-4 (2008)