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*link will take you to our exclusive distribution partner site
The SensiMix™ II Probe Kit has been developed for highly accurate real-time PCR and has been validated on all real-time PCR platforms and includes a separate vial of the passive reference dye ROX for normalization of well-to-well differences, if required.
A combination of the latest advances in buffer chemistry and PCR enhancers with a chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix II Probe Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions. The kit has been formulated for use with probe-detection technology, including hydrolysis probes (e.g. TaqMan®), displacement probes (e.g. Molecular Beacons, Scorpion®) and hybridization probes (e.g. FRET), delivering superior performance in gene expression analysis and multiplexing.
The SensiMix II Probe Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.
Broad dynamic range SensiMix II Probe Kit.
Amplification curves were obtained for the B2MG gene over 10 log dilution series. Reactions were in triplicate (average shown), using standard manufacturers’ reaction conditions. The results demonstrate that SensiMix II Probe (red) was more consistent with greater sensitivity than supplier B (green) and supplier T.
Standard and fast reaction conditions.
A standard curve obtained for -actin and -actin genes over 10 log dilution series in triplicate using standard manufacturers’ standard and fast reaction conditions. The results demonstrate SensiMix II Probe (red) gives earlier Ct values and greater sensitivity than supplier Q (black).
Reagent |
500 reactions |
SensiMix™ II Probe No-ROX (2x) |
10 x 1.25 mL (12.5 mL) |
25 µM ROX Dye |
500 µL |
BIO-83005: 500 x 50µL Reaction: 10 x 1.25 mL
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
SensiMix II Probe Kit is shipped on dry/blue ice