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    Cat. No.
    Size
    List Price*
    QTY
    BIO-33066
    50-100 Lanes
    £104.50
    +
    *To check your pricing please Login or contact us

    Description

    A broad range prestained protein marker containing 9 purified recombinant proteins with molecular weights ranging from 10 kDa to 190 kDa, for easy size determination of proteins in SDS-PAGE and transfer to a membrane.

    Product Highlights

    • Easy size determination - 9 prestained bands
    • Broad range - 10 to 190 kDa
    • Multi-colored - for easy identification and orientation
    • Easy-to-use - giving clear and sharp band resolution

    Product Description

    HyperPAGE is a broad range protein marker containing 9 purified prestained recombinant proteins with molecular weights ranging from 10 kDa to 190 kDa, ideal for molecular weight determination for a wide range of expressed proteins. The proteins are prestained and resolve into 4 blue and 5 pink bands with uniform intensity, for easy visualization and orientation.

    HyperPAGE is suitable to monitor the efficiency of electrophoretic migration in SDS-PAGE and the transfer of proteins from gel to membrane in western blotting without the addition of dye. The HyperPAGE proteins have been labeled with fluorescent dyes and are supplied lyophilized, containing the buffer salts and detergent found in the typical SDS-PAGE buffer system.

    Applications

    • Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
    • Monitoring of protein transfer onto membranes during western blotting.
    • Sizing of proteins on SDS-polyacrylamide gels and western blots.
    Main
    Molecular weight marker for easy size determination of proteins in SDS-PAGE.

    Resources

    Certification of Analysis (COAs)

    Specification

    Components

    Reagent

    50-100 lanes

    HyperPAGE Prestained Protein Marker

    500 µL

    Storage & Stability

    All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

    Once reconstituted with water the product is stable for three months at -20°C.

    Shipping conditions

    Freeze-dried and shipped on blue ice.



    FAQs

    Prestained proteins may display a different mobility in different SDS-PAGE buffer systems. Each buffer system has a slightly different pH which affects the charge of a protein and its binding capacity for SDS. However, HyperPAGE is suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. For accurate sizing, the use of unstained markers is recommended.