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    Cat. No.
    Size
    List Price*
    QTY
    BIO-65042
    30 Reactions
    £104.50
    +
    BIO-65043
    100 Reactions
    £288.00
    +
    *To check your pricing please Login or contact us

    Description

    The Tetro cDNA Synthesis Kit contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA library construction and the production of templates for PCR amplification.

    Product Highlights

    • Highly sensitive -for high-quality, full length cDNA from as little as 10 pg of total RNA
    • Ultra-stable reverse transcriptase -for long genes and rare transcripts
    • Simple -one-tube, one-step set-up for highly sensitive PCR
    • Broad dynamic range -10 pg to 2 μg of RNA

    Product Description

    The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants and the generation and cloning of cDNA.

    The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10 pg-2 μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.

    Applications

    • Construction of cDNA libraries
    • 2-step PCR assays
    • Generation of probes for hybridization
    • Gene cloning
    Main

    Specification

    Components

    Reagent

    30 reactions

    100 reactions

    5x RT Buffer

    120 µL

    400 µL

    Reverse Transcriptase (200 u/µL)

    30 µL

    100 µL

    RNase Inhibitor (10 u/µL)

    30 µL

    100 µL

    dNTP Mix 10 mM Total

    30 µL

    100 µL

    Oligo (dT)18 Primer Mix

    30 µL

    100 µL

    Random Hexamer Primer Mix

    30 µL

    100 µL

    DEPC-treated Water

    1.8 mL

    1.8 mL

    Storage & Stability

    All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.

    Shipping conditions

    Tetro cDNA Synthesis Kit is shipped on dry/blue ice



    FAQs

    For SensiFAST cDNA Synthesis Kit: The TransAmp Buffer already contains reverse transcriptase enhancers that reduce complex RNA secondary structure. For templates that have a high degree of structure, such as viral RNA and some plant RNA, we suggest using an additional 15 minute 48°C incubation step as mentioned in the manual. For Tetro cDNA Synthesis Kit: For samples with high secondary structure we suggest to denature the RNA template with primers prior to the reaction set-up. Furthermore, it is necessary to optimize the incubation time and temperature. The temperature of the RT step may be raised up to a maximum of 48°C.

    The necessary amount of cDNA template for PCR or qPCR reactions may vary from the range of pg up to several hundred ng. It depends among other things on the analyzed target, the used application and sample characteristics. If you do not have empirical values for your assays please use the recommended amounts in the manuals of your PCR or qPCR assays and optimize if necessary.

    It is not possible for the SensiFAST cDNA Synthesis Kit. Random Hexamer and oligo dT primer are already included in the optimized TransAmp buffer. It is possible to use gene specific primer for the Tetro cDNA Synthesis Kit to generate cDNA for specific target.

    We recommend to use our EPIK miRNA assays to generate and analyze cDNA from miRNA. One advantage of the EPIK assays are the 3 miRNA specific primer used for cDNA synthesis and detection. They improve the specificity and enhance the discrimination of even closely related targets.

    The SensiFAST cDNA Synthesis Kit has been designed for rapid generation and unbiased coverage of cDNA, especially for subsequent quantification with real-time PCR. The optimized 5x TransAmp buffer includes all necessary components for cDNA synthesis, including a unique blend of oligo dT and random hexamer primers and the RiboSafe RNase Inhibitor. The Tetro cDNA Synthesis Kit is the more flexible solution. You selects the desired setup, especially the desired RT primer. The Tetro Kit is optimized to work over a wide range of RNA concentrations and to obtain full length cDNA, which can be used for two-step PCR assays as well. Tetro is the recommended Kit for the generation of cDNA libraries and cloning.

    Reviews

    "Having used the Bioline Tetro cDNA Synthesis Kit and SensiFast SYBR and Probe mi ..."

    "We started to use this kit in our lab for all our routine cDNA synthesis and we ..."