HyperLadder™ 50bp

Cat No. Size List Price* Qty  
BIO-33054 100 Lanes
$61.00
 
BIO-33039 200 Lanes
$96.00
 
BIO-33040 500 Lanes
$223.00
 
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HyperLadder™ 50bp is a ready-to-use molecular weight marker, especially designed for easy size determination.

The ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder™ 50bp from the vial to the gel.

Features & Benefits

  • Easy size determination - 15 bands from 50bp - 2000bp
  • Contains dye - for direct gel loading
  • Several higher intensity bands - for easy orientation
  • Easy storage - stable for 6 months at room temperature

Description

HyperLadder™ 50bp is a ready-to-use molecular weight marker, specially designed for easy size determination. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 50bp from the vial to the gel.

HyperLadder 50bp produces a pattern of 15 regularly spaced bands, ranging from 50 to 2000bp. To allow easy identification and orientation, the 300, 1000 and 2000bp bands have the highest intensity. The double-stranded ladder can be visualized on 2% to 3% agarose gels after ethidium bromide staining.

Optionally the HyperLadder can be used for mass determination. A 5x sample loading buffer is supplied for your convenience. See our HyperLadder Selection Guide page for product comparisons of the complete HyperLadder range.

For best results when using our HyperLadder range we recommend using Bioline Agarose or Bioline Agarose Tablets.


Components

Reagent

100 Lanes

200 Lanes

500 Lanes

HyperLadderTM 50bp

500µl

2 x 500µl

5 x 500µl

5x Sample Loading Buffer

1ml

1ml

1ml


Storage & Stability

All components are shipped at room temperature or on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended.

When stored at +4°C, reagents will remain stable for a period of 12 months.

When stored at room temperature, reagents will remain stable 6 months from date of receipt.


Shipping conditions

Ambient temperature.


Certificates of Analysis (COAs)

Certificates of Analysis for HyperLadder™ 50bp are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 10 COAs for HyperLadder™ 50bp →


Frequently asked questions

  • What are the optimal conditions for running my HyperLadder?

      Please see individual product insert available online and included with each product for the concentration of Agarose each ladder should be run on. The smaller the DNA product, the higher the percentage of Agarose required in the gel. All HyperLadders are ready-to-load, and require no addition of loading buffer prior to use.
  • My HyperLadder doesn't look as it should when I run it, what should I do?

      Problems with our HyperLadders are extremely rare, so please make sure you have carried out each of the following correctly:
      • Used the correct percentage of Agarose in your gel for the specific marker in question.
      • Used 5µl of the marker per lane.
      • Used the correct concentration of stain (e.g. EtBr) to visualise correctly.
      • Vortexed the marker gently before use.
      Should problems persist, please contact technical services for further assistance.
  • Do I need to pre-treat my HyperLadder prior to loading?

      No pre-treatment, such as denaturing, is necessary prior to use.
  • Are your ladders suitable for use in Polyacrylamide Gel Electrophoresis (PAGE)?

      Our HyperLadder range is designed for use on Agarose gels, and we do not recommended them for PAGE. However, should you wish to use Bioline HyperLadders in this technique, HyperLadder™ 25bp, our small fragment DNA marker, would be the most appropriate.
  • We do not wish to stain our gels using EtBr, will other stains still resolve the HyperLadder correctly?

      Other stains, such as SYBR® Green Gel Stain, can be used with our HyperLadders as effectively as EtBr. The fluorescence of the gel as a whole may vary depending on the stain used, however the relative intensities of the marker bands and of the samples run will remain the same, thus the HyperLadder remains quantitative.
  • Do I need to add loading buffer to my Bioline marker to load it onto my gel?

      No, our Ladder range is supplied in a ready-to-load format to be directly loaded onto your agarose gel. An additional vial of 5x Loading Buffer is supplied with each of our markers for use with your samples.



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