MyFi™ DNA Polymerase

Cat No. Size List Price* Qty  
BIO-21117 250 Units
$200.00
 
BIO-21118 500 Units
$357.00
 
BIO-21119 2500 Units
$1,672.00
 
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*For more information on pricing please contact us

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MyFi™ is a novel, antibody-mediated DNA polymerase complex with enhanced sensitivity and fidelity, ideally suited to problematic DNA templates.

Features & Benefits

  • New generation DNA polymerase complex - provides robust PCR with 3.5-fold higher fidelity than wild-type Taq polymerase
  • Antibody-based hot-start polymerase - enhances specificity and allows room-temperature reaction set-up
  • High processivity - efficiently amplifies complex mammalian DNA fragments up to 10kb
  • Novel 5x buffer system - ensures reliability, reproducibility and convenience

Applications

  • Routine PCR applications
  • Specifically developed for TA cloning
  • High throughput
  • Good for low copy PCR assays
  • PCR requiring high specificity combined with higher fidelity

Description

MyFi DNA Polymerase is a high-performance, new generation DNA polymerase complex, combining the sensitivity of our ever-popular MyTaq HS DNA Polymerase with a highly processive proof-reading DNA polymerase. This unique combination coupled with an efficient antibody-based hot-start is engineered and optimized for maximum sensitivity and robust, high-fidelity PCR amplification.

MyFi has been developed with an industry-leading, novel buffer chemistry containing pre-optimized concentrations of ultra-pure dNTPs, MgCl2 and proprietary PCR enhancers for exceptional versatility across a range of challenging assays including long PCR, low-copy PCR and amplification of problematic GC and AT-rich genomic templates (Fig. 1).

MyFi DNA Polymerase generates extremely high DNA yields and produces 3'-A overhangs which are ideal for direct integration into TA-cloning vectors. In addition, MyFi provides the convenience of room temperature reaction assembly and avoids non-specific amplification and primer-dimer formation.


Notes

MyFi is a Trademark of Bioline.


Components

Reagent

250 Units

500 Units

2500 Units

MyFi DNA Polymerase

1 x 125µl

1 x 250µl

2 x 625µl

5x MyFi Reaction Buffer

1 x 625µl

1 x 1.25ml

5 x 1.25ml


Volume

BIO-21117: 125 x 25µl Reactions: 1 x 125µl

BIO-21118: 250 x 25µl Reactions: 1 x 250µl

BIO-21119: 1250 x 25µl Reactions: 2 x 625µl


Concentration

2u/µl


Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

On Dry Ice or Blue Ice.




Customer Testimonials

We commonly use the Bioline MyFi polymerase, and it works better than any other polymerase kit. The MyFi polymerase could amplify complicated short tandem repeat (STR or SSR/microsatellite) sequences better than the other kits. In the figure, the MyFi kit could amplify the STR-A sequence, which the phusion kit could not. The STR-B sequence was amplified with less non-specific amplicons when the MyFi kit was used.
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I like the Bioline MyFi polymerase, as it can amplify any sequence even if the fragment size is over 3 kb. The polymerase is also useful for cross-species amplification. The figure shows amplification results from meadow fescue using PCR primers designed for perennial ryegrass. All 5 gene sequences were successfully amplified by the cross-species PCR, even though the largest fragment (Gene 4) was over 3 kb in length.
"For verification of difficult to detect genes this product worked great, whereas my usual PCR reagents failed to amplify anything out of my samples."
"MyFi Taq allowed the same result with half of the sample and half of the time."
"MyFi gave best results with a long AT-rich template and two-step PCR using poorly matched primers."
"MyFi was able to produce high yield clean PCR product when Roche high fidelity plus PCR system failed to produce any product or only low yield."

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