MyTaq™ HS Red DNA Polymerase

Cat No. Size List Price* Qty  
BIO-21115 1000 Units
$531.00
 
BIO-21116 2500 Units
$1,198.00
 
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MyTaq™ HS Red DNA Polymerase is a red-colored, high performance PCR reagent that is powered by antibody-mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. MyTaq HS Red does not exhibit polymerase activity during the reaction set-up, thus reducing non-specific amplification including primer-dimer formation.

Features & Benefits

  • Universal DNA polymerase - recommended for most applications
  • Fast - complete PCR reaction in less than 30 minutes
  • Antibody-based hot-start polymerase - for highest specificity
  • Superior performance - highly optimized buffer system, including ultra-pure dNTPs and MgCl2
  • Red dye - for direct gel loading
  • All-in-one mastermix available- for enhance reproducibility

Applications

  • Fast PCR reactions
  • Colony PCR
  • Genotyping
  • Multiplexing
  • Low-copy PCR assays
  • Specific amplification of difficult templates (GC-rich)
  • Assays with prolonged reaction set-up

Description

MyTaq™ HS Red uses the latest technology in PCR enzyme design, engineered to increase affinity for DNA, resulting in significant improvements in yield, sensitivity and speed (fig. 1).

MyTaq HS Red has the added convenience of room temperature reaction assembly, thus avoiding non-specific amplification and primer-dimer formation. With activation times as low as 1 to 2 minutes, a complete PCR assay can be completed in less than 30 minutes. The enzyme is supplied with an industry-leading novel buffer system, consisting of proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations, this removes the need for optimizing individual reactions and delivers superior amplification time after time (fig. 2).

With an inert red dye, samples can be loaded directly onto the agarose gel without the need for an additional loading buffer, as the mix is of sufficiently high density to sink to the bottom of the well, making MyTaq HS Red DNA Polymerase ideal for high throughput assays.


Notes

MyTaq is a trademark of Bioline.


Components

Reagent

250 Units

1000 Units

2500 Units

MyTaq HS Red DNA Polymerase

1 x 50µl

1 x 200µl

2 x 250µl

5x MyTaq Reaction Buffer

2 x 1ml

8 x 1ml

14 x 1.5ml


Concentration

5u/μl


Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

On Dry Ice or Blue Ice.


One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.


Certificates of Analysis (COAs)

Certificates of Analysis for MyTaq™ HS Red DNA Polymerase are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 23 COAs for MyTaq™ HS Red DNA Polymerase →

Citations

  1. Impacts of climatic factors on evolution of molecular diversity and the natural distribution of wild stocks of the giant freshwater prawn (Macrobrachium rosenbergii)
    Hurwood, D.A., Dammannagoda, S., Krosch, M.N., Jung, H., Salin, K.R., Youssef, M.B., de Bruyn, M., Mather, P.B.

Frequently asked questions

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Customer Testimonials

I like this product because it is easy to use and fast to set up a PCR reaction. Very convenient! I used it for regular PCR and it worked well to amplify my product. It also has a red mix, so after PCR I don't have to add any loading buffer, which is awesome!
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"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."

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