- Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
- Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
- Specific – MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
- Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
- Convenient – buffer system includes a red dye for improved pipetting ease / accuracy and to enable direct gel loading
- Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
- Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
MyTaq™ HS Red DNA Polymerase is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. The MyTaq HS DNA Polymerase and MyTaq Red Reaction Buffer in this product, are a unique combination of next-generation hot-start DNA polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS has been developed to give more robust amplification than other commonly-used polymerases meaning it performs reliably even in the presence of PCR inhibitors
MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme, specifically designed for highly-specific, efficient amplification from even the most challenging templates. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start. Furthermore, the advanced formulation of MyTaq HS and buffer system allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield.
The enzyme is supplied with a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq Red Reaction Buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.
- Fast PCR
- Multiplex PCR
- Specific amplification of challenging (e.g. GC-rich) templates
- Multiplex PCR
- Colony PCR
- Low copy number PCR assays
- High-throughput assays with prolonged PCR set-up
Introduction to MyTaq
Overview, features and benefits of the MyTaq product family
PCR Enzyme GuideDownload the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research
PCR Selection ChartSelect the best reagent for your research
Application NoteMyTaq™ HS Red DNA Polymerase
I like this product because it is easy to use and fast to set up a PCR reaction. Very convenient! I used it for regular PCR and it worked well to amplify my product. It also has a red mix, so after PCR I don't have to add any loading buffer, which is awesome!
Kristina Marinak, West Virginia University, Morgantown, US
Product SelectionPlease refer to the PCR Selection Chart to confirm the recommended product for your PCR application.
Certification of Analysis (COAs)
MyTaq HS Red DNA Polymerase
1 x 50 µL
1 x 200 µL
2 x 250 µL
5x MyTaq Reaction Buffer
2 x 1 mL
8 x 1 mL
14 x 1.5 mL
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
On Dry Ice or Blue Ice.
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.
MyTaq is a trademark of Bioline.
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
|No or low PCR yield||Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.|
|Primers degraded – check quality and age of the primers.|
|Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM.|
|Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration.|
|Template concentration too low – Increase concentration of template.|
|Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.|
|Multiple Bands||Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.|
|Prepare master mixes on ice or use a heat-activated polymerase.|
|For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.|
|Smearing or artifacts||Template concentration too high. Prepare serial dilutions of template.|
|Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.|
|Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.|
|Extension time too long. Reduce extension time in 0.5-1 minute increments.|