A new generation of polymerase that delivers improved yield, sensitivity, speed and robustness when amplifying targets from any template.
MyTaq™ was compared with DNA polymerases form others suppliers for the amplification of a 450 bp fragment of the human myc gene (61% GC rich). Decreasing amounts of human genomic DNA were used as template (1 mg, 200 ng, 100 ng, 50 ng, 25 ng and 12.5 ng; lanes 1-6 respectively) in the PCR. Marker is HyperLadder 1kb (M). MyTaq™ delivers higher yield and sensitivity compared to five polymerases from alternative suppliers.
A 450 bp fragment of the human myc gene (61% GC rich) was amplified under fast conditions using MyTaq™ with a DNA polymerase from supplier S. The PCR was performed using both enzymes and a three-fold decreasing amount of human genomic DNA as template (200 ng – 30 pg; lanes 1-8 respectively). Marker is HyperLadder 1kb (M). MyTaq™ amplifies the target more efficiently under fast conditions, resulting in higher yield, without the need for further optimization.
MyTaq™ DNA Polymerase is recommended for all standard PCR applications. The MyTaq™ DNA Polymerase and MyTaq™ Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq™ has an increased affinity for DNA, enabling reliable amplification from very low amounts of template. MyTaq™ DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors.
The composition of the buffer system is critical for efficient PCR. MyTaq reaction buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, saving time, effort and the cost of performing unnecessary assay repeats.
The combination of MyTaq™ and optimized buffer system allow for faster PCR reactions compared with other polymerases, therefore reducing overall run time from approximately 1 hour to under 30 minutes. This is achieved without compromising specificity or yield, reducing the reaction time allows for increased throughput and faster time to results.
| Presentation | BIO-21105 500 Units (Polymerase 100 µL, Buffer 4x 1 mL) BIO-21106 2,500 Units (Polymerase 2x 100 µL, Buffer 14x 1.5 mL) BIO-21107 5,000 Units (Polymerase 4x 100 µL, Buffer 9x 5 mL) |
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| Appearance | Clear, colorless solutions | ||
| Hot start | No | ||
| Application | Standard PCR, High-yield PCR, Fast PCR, Colony PCR, TA cloning | ||
| Sample type | DNA, cDNA | ||
| Presentation | 5 vials / 16 vials / 13 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Specific Activity | 5 u/µL | ||
| Functional | Single band of PCR product on an agarose gel | ||
| DNA Contamination | No detectable product in a qPCR assay | ||
| DNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-21105 500 Units
BIO-21106 2500 Units
BIO-21107 5000 Units
Monica Kiela, University of Arizona, Tucson, US
Robert Gordon University, Aberdeen, Scotland, UK
Arbovax, Inc., USA
Monica Kiela, University of Arizona, Tucson, US
Uni Lübeck, Germany
Brigham Young University, USA
Cornell University, USA
San Diego Zoo, USA
Stirling University, Scotland, UK
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