Simple, efficient membrane-based method for the purification of DNA from PCR reactions and from TAE & TBE agarose gels, without the need for hazardous reagents or
5 kb, 1.2 kb and 500 bp PCR fragments were run on an agarose gel (lanes 1, 3 and 5) alongside the same products purified with ISOLATE II PCR and Gel Kit (lanes 2, 4 and 6). The results illustrate the ability of the ISOLATE II PCR and Gel Kit to remove small contaminants such as primers, primer-dimers, enzymes etc. without loss of the PCR product.
5 kb, 1.2 kb, 500bp and 100 bp PCR fragments were run on an agarose gel (lanes O) alongside the same products run on 1% TAE agarose gel and extracted from the gel using ISOLATE II PCR and Gel Kit (lanes P). The results illustrate very high recovery rates of DNA fragments from agarose gels.
ISOLATE II PCR and Gel Kit is the simplest option for the purification of PCR products and for the isolation of DNA fragments from TAE and TBE agarose gel slices. A fast and easy-to-follow protocol is given for each application.
PCR products can be purified in 10 minutes using simple binding and elution steps. Concentrated PCR products ranging between 60 bp and 15 kb can be eluted, removing primers, nucleotides, enzymes, mineral oil, salts and other impurities.
DNA fragments between 50 bp and 20 kb can be extracted from agarose gel slices in 20 minutes using a color indicator to help maintain optimal pH and identify undissolved agarose.
ISOLATE II PCR and Gel Kit has been designed to deliver optimal performance in downstream applications, including transformations, cloning, sequencing and restriction analysis.
| Presentation | 10 Preps | 50 Preps | 250 Preps |
| ISOLATE II PCR and Gel Columns (yellow) | 10 | 50 | 250 |
| Collection Tubes | 10 | 50 | 250 |
| Binding Buffer CB | 10 mL | 40 mL | 200 mL |
| Wash Buffer CW (Concentrate) | 6 mL | 25 mL | 2×50 mL |
| Elution Buffer C | 13 mL | 13 mL | 30 mL |
| Appearance | Colorless / Binding Buffer CB – blue | ||
| Application | Cloning, Ligation, Restriction digestion, Fluorescence sequencing, Labeling, PCR, Transfection, In vitro transcription | ||
| Sample type | DNA from enzymatic reactions and TAE or TBE agarose gels | ||
| Preparation time | 10 min for 6 PCR purifications, or 20 min for 6 gel extractions | ||
| DNA size distribution | 50 bp to ≥ 20 kb | ||
| Elution volume | 15 – 30 µL | ||
| Yield | Up to 25 µg of DNA | ||
| Storage | All kit components at room temperature (18-25°C) | ||
| Functionality | Nucleic acid recovery >90% | ||
Cat. No. Size
BIO-52058 10 Preps
BIO-52059 50 preps
BIO-52060 250 preps
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