To complement the SensiFAST™ Probe and SYBR® kits, Meridian has developed the SensiFAST cDNA Synthesis Kit.
SensiFAST cDNA Synthesis Kit and a kit from supplier B were used for reverse transcription of a specific target from total RNA under fast conditions. The results illustrate that the SensiFAST cDNA Synthesis Kit (red) is more sensitive than the kit from supplier B (blue), as judged by the lower Ct values.
SensiFAST cDNA Synthesis Kit was employed in 48 independent first-strand reactions, containing 100 ng total RNA and another 48 reactions containing 100 pg. The resultant first-strand product from each RT reaction was used in triplicate qPCR assays. The results demonstrate the excellent reproducibility of SensiFAST cDNA Synthesis Kit at high and low input RNA, as judged by the same Ct values.
SensiFAST cDNA Synthesis Kit and a kit from supplier B were for reverse transcription of a specific target from total RNA. Primer pairs were then designed against the transcript at 1 kb intervals and used in qPCR reaction with SensiFAST SYBR® No-ROX. The consistent Ct values show that, unlike the results from supplier B (blue), the SensiFAST cDNA Synthesis Kit (red) did not show any bias across the target.
SensiFAST cDNA Synthesis Kit was used for reverse transcription of a specific target from a 10-fold serial dilution of total RNA, for either 5 min (blue) or 60 min (red) at 42 °C. The results illustrate the high efficiency of reverse transcription over a broad linear dynamic range under both standard and fast conditions.
The SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies.
SensiFAST cDNA Synthesis Kit contains a highly-pure reverse transcriptase and optimized TransAmp™ buffer system, which includes a unique blend of random hexamers and anchored oligo (dT) primers to deliver the highest quality qPCR ready cDNA. This makes the SensiFAST cDNA Synthesis Kit ideal for working with limited sample volumes, such as laser-micro dissected samples and tissue biopsies (down to 1 pg of input RNA), to reverse transcribe precious RNA into stable cDNA ready for accurate real-time quantification. The unique blend of random hexamer primers and anchored oligo dT in the TransAmp Buffer also ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions.
SensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SensiFAST SYBR® Kits for fast real-time RT-qPCR without compromising on quality, giving real-time results in less than an hour.
| Presentation | BIO-65053 50 Reactions (RT 50 µL, Buffer 200 µL) BIO-65054 250 Reactions (RT 250 µL, Buffer 1 mL) |
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| Appearance | Clear, colorless solutions | ||
| Application | Gene expression analysis, tissue biopsy analysis, miRNA profiling / quantification, RNA target detection, pathogen detection | ||
| Sample type | Total RNA or mRNA | ||
| Presentation | 2 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Functional | qPCR tested product | ||
| DNA Contamination | None detected in PCR amplification with traces overlay with the negative control on E. coli and mouse genomic DNA specific targets. | ||
| DNase Contamination | No detectable degradation | ||
| RNase Contamination | No detectable activity | ||
Cat. No. Size
BIO-65053 50 Reactions
BIO-65054 250 Reactions
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