Tetro™ cDNA Synthesis Kit contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers
A ten-fold serial dilution of RNA (1 μg to 100 pg) was reverse transcribed using Tetro Reverse Transcriptase and oligo (dT)18. The resultant cDNA was then used as template in a PCR using primers for amplification of a 700 bp fragment from mouse b-actin. Lanes 1-5 correspond to PCR product from the serial dilution above, reactions were carried out in duplicate. HyperLadder 50bp (M).
A ten-fold serial dilution of RNA (1 μg to 10 pg) was reverse transcribed using Tetro Reverse Transcriptase and oligo (dT)18. The resultant cDNA was then used as a template in a PCR using primers for amplification of a 470 bp fragment from human GAPDH. Lanes 1-5 correspond to PCR product from the serial dilution above, reactions were carried out in duplicate. HyperLadder 50bp (M).
Tetro™ cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants and the generation and cloning of cDNA.
Tetro™ cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10 pg-5 μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.
| Presentation | BIO-65042 30 Reactions (RT 30 µL, Buffer 120 µL, RI 30 µL, dNTPs 30 µL, Oligo primer 30 µL, Random hex primer 30 µL, Water 1.8 mL) BIO-65043 100 Reactions (RT 100 µL, Buffer 1.2 mL, RI 100 µL, dNTPs 100 µL, Oligo primer 100 µL, Random hex primer 100 µL, Water 1.8 mL) |
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| Appearance | Clear, colorless solutions | ||
| Application | Construction of cDNA libraries, 2- Step qPCR assays, generation of probes for hybridization, gene cloning | ||
| Sample type | Total RNA or mRNA | ||
| Presentation | 7 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Specific Activity | RT 200 u/µL, RI 10 u/µL, dNTP Mix 10 mM (total) | ||
| Functional | Detection of cDNA on an agarose gel | ||
| Endonuclease contamination | None detected with super coiled DNA plasmid incubated with the RT for 1 hour at 37°C | ||
| DNase Contamination | No detectable degradation | ||
| RNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-65042 30 Reactions
BIO-65043 100 Reactions
Kelly Condon, James Cook University, College of Public Health, Townsville, Australia
Patricia Rueda, Monash Institute of Pharmaceutical science, Parkville, Australia
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