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EasyLadder I

Ready-to-use molecular weight marker, designed for fast size determination of nucleic acids from 100 bp to 2000 bp.

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EasyLadder I

Broad size range - 5 evenly distributed bands from 50 bp to 2000 bp

Ready-to-use - reduces handling steps and saves time

Fast separation - ideal for PCR products

Supplied with loading dye - for loading sample DNA

Product Description

Agarose gels are often run to check and see if a band is present or not, for example in a PCR reaction.  With 5 regularly spaced bands, ranging from 100 bp to 2000 bp, EasyLadder™ I is a molecular weight marker especially designed for fast size determination of linear double-stranded DNA fragments on 0.5% to 3% TAE or TBE agarose gels.

This ready-to-use format, with a red loading dye already added to the ladder, reduces handling steps and saves time; simply transfer EasyLadder I from the vial to the gel, along with the PCR product and run at high voltage for just a few minutes (1-3 cm of a gel lane). The concentration of DNA in each of the EasyLadder I bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The EasyLadder I is also supplied with a 5x loading dye for the loading of sample DNA.

Applications

  • Determination of PCR amplicon size
  • Confirmation of plasmid construction
  • Restriction fragment size determination

Product Specification

Presentation BIO-33045 200 Lanes (Ladder 2x 500 µL, Buffer 1 mL)

BIO-33046 500 Lanes (Ladder 5x 500 µL, Buffer 1 mL)
Appearance Blue solution
Markers 5 regularly spaced bands, ranging from 50 to 2000 bp
Application Restriction fragment mapping, Sequence analysis, Confirmation of plasmid construction, Detection of DNA polymorphisms, Southern/Northern blotting, Cloning
Presentation 3 vials / 6 vials
Storage 4 °C to -20 °C
Mix stability See outer label
Functional Each band sequenced and visual compared to standard

Product Ordering

Cat. No.              Size

BIO-33045           200 Lanes

BIO-33046           500 Lanes

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FAQs:

EasyLadder I

What are the optimal conditions for running my ladder?
All HyperLadders and EasyLadders are ready-to-load and require no addition of loading buffer prior to use. In general, the smaller the DNA product, the higher the percentage of Agarose required in the gel. As a guide for optimization we would suggest to use the EasyLadder I with 2% (w/v) agarose gel, Hyperladder 100bp with 2% (w/v) agarose gel, Hyperladder 1kb with 1% (w/v) agarose gel, Hyperladder 50bp with 1.5% (w/v) agarose gel and Hyperladder 25bp with 3.5% (w/v) agarose gel. Please have a look into the related application note for more information about ladder migration in different gel concentrations.
My ladder does not look as it should when I run it, what should I do?
Problems with our HyperLadder and EasyLadder are extremely rare, so please make sure you have carried out each of the following correctly: - Used the correct percentage of agarose in your gel for the specific marker in question. - Used 5 µL of the marker per lane. - Used the correct concentration of stain (e.g. EtBr) to visualise correctly. - Vortexed the marker gently before use. Should problems persist, please contact technical services for further assistance.
Do I need to pre-treat my HyperLadder / EasyLadder prior to loading?
No pre-treatment, such as denaturing, is necessary prior to use.
Do I need to add loading buffer to my Meridian marker to load it onto my gel?
No, our Ladder range is supplied in a ready-to-load format to be directly loaded onto your agarose gel. An additional vial of 5x Loading Buffer is supplied with each of our markers for use with your samples.
Are your ladders suitable for use in Polyacrylamide Gel Electrophoresis (PAGE)?
Our HyperLadder and EasyLadder range is designed for use on Agarose gels and we do not recommend them for PAGE. However, should you wish to use Meridian HyperLadders in this technique, HyperLadder 25bp, our small fragment DNA marker, would be the most appropriate.
We do not wish to stain our gels using EtBr, will other stains still resolve the HyperLadder / EasyLadder correctly?
Other stains, such as SYBR® Green Gel Stain, can be used with our ladders as effectively as EtBr. The fluorescence of the gel as a whole may vary depending on the stain used, however the relative intensities of the marker bands and of the samples run will remain the same, thus the HyperLadder / EasyLadder remains quantitative.
What are the conditions of the gel image in the product insert?
EasyLadder I: gel with 2% (w/v) agarose in TBE HyperLadder 1kb: gel with 1% (w/v) agarose in TBE HyperLadder 100bp: gel with 2% (w/v) agarose in TBE HyperLadder 50bp: gel with 1,5% (w/v) agarose in TBE HyperLadder 25bp: gel with 3,5% (w/v) agarose in TBE For all 5 µL ladder have been applied and visualized using ethidium bromide.
Which kind of tracking dye does the ladder and the related loading buffer contain?
The EasyLadder and the related red DNA loading buffer contain cresol red. The HyperLadder and the related blue DNA loading buffer contain bromophenol blue.

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