A convenient mastermix containing a new generation of hot-start polymerase that delivers improved specificity, yield, speed and robustness when amplifying targets from any template.
A 340 bp (A) and a 450 bp (B) fragment of the myc gene, a 525 bp (C) fragment of the EGFR gene and a 530 bp (D) fragment of the AGRI1 gene were amplified using MyTaq™ HS and hot-start DNA polymerases from Suppliers F, K, G and S. Each polymerase was used to set-up PCR reactions containing either 100 ng, 33 ng, 10 ng, 4 ng, 1 ng, 33 pg, 10 pg and 3 pg of human genomic DNA (Lanes 1-8 respectively), prepared by a 3-fold serial dilution. Marker is HyperLadder 1kb (M). MyTaq™ HS performed well across all four human genes.
M13 vector carrying a DNA insert was cloned into E.coli cells. 2 µL increments of agar (2a) and 2 µL increments of LB (2b) were added to a series of 50 µL PCR reactions (Lanes 1-8 respectively). The results show that MyTaq™ HS DNA Polymerase was more resistant to inhibition than that of supplier S. Individual colonies were picked from a plate of E.coli, washed directly into MyTaq™ buffer and amplified using MyTaq™ HS and primers for either a 2.6 kb or an 884 bp amplicon. Marker is HyperLadder 1kb (M). The results illustrate MyTaq™ is robust and gives highly–specific results.
MyTaq™ HS Mix is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. MyTaq™ HS Mix is comprised of MyTaq™ HS DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq™ HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq™ HS has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq™ HS means it gives excellent results under fast PCR conditions. MyTaq™ HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start.
The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up for improved speed, throughput and assay reproducibility. The inclusion of dNTPs, MgCl2 and enhancers at optimal concentrations, helps eliminate the need for optimization, thereby saving time and ensuring comparable efficiencies for annealing and extension of all primers in the reaction, this makes MyTaq™ HS Mix ideal for multiplex PCR.
| Presentation | BIO-25045: 200 x 50 µL Reactions: 4 x 1.25 mL BIO-25046: 1,000 x 50 µL Reactions: 20 x 1.25 mL |
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| Appearance | Clear, colorless solutions | ||
| Hot start | Antibody mediated | ||
| Application | Fast PCR, Multiplex PCR, Genotyping, Complex templates (e.g. GC-rich), Low copy number PCR assays, High-throughput assays with prolonged PCR set-up | ||
| Sample type | DNA, cDNA | ||
| Presentation | 4 vials / 20 vials | ||
| Storage | -20 °C | ||
| Mix stability | See outer label | ||
| Specific Activity | 2x | ||
| Functional | Single distinct bands of PCR product on an agarose gel and sensitivity | ||
| DNA Contamination | No detectable product in a qPCR assay | ||
| DNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-25045 200 x 50µl Reactions
BIO-25046 1000 x 50µl Reactions
Albert Perez, Moores Cancer Center - UCSD, La Jolla, US
Roslin Institute, Scotland
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