A highly inhibitor-resistant qPCR master mix that provides quick and easy extraction and amplification of DNA from a variety of tissue types. The supermix maximizes sensitivity
20% human whole blood containing anticoagulants (EDTA, Heparin and Citrate) and bovine whole blood (EDTA), human cerebrospinal fluid, human urine and cow whole milk were analyses along with 2% alkaline or proteinase K mouse tail, liver and brain crude DNA extracts. The results illustrate that the reaction efficiency of the SensiFAST Direct Probe SuperMix remained within tolerances (90-110%) in the presence of a wide range of common PCR inhibitors.
A 10-fold serial dilution of genomic DNA spiked into human urine was amplified, using SensiFAST Direct Probe SuperMix (green) and an Inhibitor-Tolerant Mix from supplier K (red) and the manufacturers’ recommended protocol. The results illustrate SensiFAST Direct qPCR Probe SuperMix is more sensitive than the mix from supplier K, as lower dilutions could be detected, with better efficiencies for all three inhibitor samples.
A 10-fold serial dilution of genomic DNA from mouse tail clippings was spiked proteinase K lysate and after neutralization, used with SensiFAST Direct Probe SuperMix (green) and an Inhibitor-Tolerant Mix from supplier K (red) using the manufacturers’ recommended protocol. The results illustrate SensiFAST Direct qPCR Probe SuperMix is more sensitive than the mix from supplier K, particularly with the proteinase K lysate, as lower dilutions could be detected, with better efficiencies.
SensiFAST™ Direct Probe SuperMix is a combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an antibody-mediated hot-start polymerase, dNTPs and MgCl2. It has been designed for highly reproducible, accurate assay results in the presence of inhibitors, making it ideal for direct amplification directly from the most challenging samples.
The advanced buffer chemistry and enhancers has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe‑detection technology, including TaqMan®, Scorpions® and molecular beacon probes, making SensiFAST Direct Probe SuperMix perfect for multiplexing, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.
| Concentration | 2x |
| Presentation | BIO-86105: 500 x 20 µL Reactions: 5 x 1 mL BIO-86120: 2000 x 20 µL Reactions: 4 x 5 mL |
| Appearance | Clear, colorless solution |
| Hot Start | Antibody mediated |
| Application | Probe-based, qPCR, two-step RT-qPCR |
| Sample type | cDNA, DNA |
| Presentation | 5 vials / 4 vials |
| Storage | -20 °C |
| Mix stability | See outer label |
| Consistency | ±0.5 Ct variance between test and reference sample |
| DNA Contamination | None detected in PCR amplification with traces overlay with the negative control on E. coli and mouse genomic DNA specific targets. |
| DNase Contamination | No detectable degradation |
Cat. No. Size
BIO-86105 500 x 20µl Reactions
BIO-86120 2000 x 20µl Reactions
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