Outstanding assay reproducibility, sensitivity and specificity for both DNA and cDNA templates.
Amplification curves were obtained for the B2MG gene over 10 log dilution series. Reactions were in triplicate (average shown), using standard manufacturers’ reaction conditions. The results demonstrate that SensiMix II Probe (red) was more consistent with greater sensitivity than supplier B (green) and supplier T.
A standard curve obtained for -actin and -actin genes over 10 log dilution series in triplicate using standard manufacturers’ standard and fast reaction conditions. The results demonstrate SensiMix II Probe (red) gives earlier Ct values and greater sensitivity than supplier Q (black).
The SensiMix™ II Probe Kit has been developed for highly accurate real-time PCR and has been validated on all real-time PCR platforms and includes a separate vial of the passive reference dye ROX for normalization of well-to-well differences, if required.
A combination of the latest advances in buffer chemistry and PCR enhancers with a chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix II Probe Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions. The kit has been formulated for use with probe-detection technology, including hydrolysis probes (e.g. TaqMan®), displacement probes (e.g. Molecular Beacons, Scorpion®) and hybridization probes (e.g. FRET), delivering superior performance in gene expression analysis and multiplexing.
The SensiMix II Probe Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.
| Concentration | 2x | ||
| Presentation | BIO-83005: 500 Reactions (Mix 10 x 1.25 mL, ROX™ dye 500 µL) | ||
| Appearance | Clear, colorless mix, pink ROX™ solution | ||
| Hot Start | Antibody mediated | ||
| Application | Probe-based, qPCR, two-step RT-qPCR, genotyping, copy number variation (CNV) analysis | ||
| Sample type | cDNA, DNA | ||
| Presentation | 11 vials | ||
| Storage | -20 °C, avoid exposure of the ROX™ to light | ||
| Mix stability | See outer label | ||
| Functional | ±0.5 Ct variance between test and reference sample | ||
| Specific Activity | ROX™ 25 µM | ||
| DNA Contamination | None detected in PCR amplification with traces overlay with the negative control on E. coli and mouse genomic DNA specific targets. | ||
| DNase Contamination | No detectable degradation | ||
Cat. No. Size
BIO-83005 500 Reactions (Mix 10 x 1.25 mL, ROX™ dye 500 µL)
Fansuo Geng, Walter and Eliza Hall Institute of Me
Yale University, Forestry and Enviromental Studies
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