- Sensitive – exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
- Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
- Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
- Flexible – ideal for amplifying any target up to 5 kb, including DNA extracted from human, animal and plant samples
- Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
- Convenient – includes all the components necessary for high performance PCR amplification
MyTaq™ DNA Polymerase is recommended for all standard PCR applications. The MyTaq DNA Polymerase and MyTaq Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors.
The composition of the buffer system is critical for efficient PCR. MyTaq reaction buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, saving time, effort and the cost of performing unnecessary assay repeats.
The combination of MyTaq and optimized buffer system allow for faster PCR reactions compared with other polymerases, therefore reducing overall run time from approximately 1 hour to under 30 minutes. This is achieved without compromising specificity or yield, reducing the reaction time allows for increased throughput and faster time to results.
- Standard PCR
- High-yield PCR
- Fast PCR
- Colony PCR
- TA cloning
We use MyTaq DNA Polymerase for our mouse colony PCR screening. My established PCR protocols almost always work, and the run is shorter. Even new PCR protocols are much easier to establish since this polymerase is more robust than many others. We found that in several instances, if nothing works, this polymerase does.
Monica Kiela, University of Arizona, Tucson, US
Product SelectionPlease refer to the PCR Selection Chart to confirm the recommended product for your PCR application.
Certification of Analysis (COAs)
MyTaq DNA Polymerase
1 x 100 µL
2 x 250 µL
4 x 250 µL
5x MyTaq Reaction Buffer
4 x 1 mL
14 x 1.5 mL
9 x 5 mL
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
On Dry Ice or Blue Ice.
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C
MyTaq is a trademark of Bioline.
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
|No or low PCR yield||Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.|
|Primers degraded – check quality and age of the primers.|
|Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM.|
|Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration.|
|Template concentration too low – Increase concentration of template.|
|Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.|
|Multiple Bands||Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.|
|Prepare master mixes on ice or use a heat-activated polymerase.|
|For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.|
|Smearing or artifacts||Template concentration too high. Prepare serial dilutions of template.|
|Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.|
|Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.|
|Extension time too long. Reduce extension time in 0.5-1 minute increments.|