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Ordering

Cat. No.
Size
BIO-32005
500 Reactions
BIO-32020
2000 Reactions

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Description

Precise, highly reproducible and fast genotyping of sequence variants through high resolution melting.

Product Highlights

  • Accurate – clear discrimination of even the most challenging sequence differences
  • Reproducible – unparalleled consistency between replicate difference plots for increased confidence in sample characterization
  • Sensitive – efficient detection from even very limiting amounts of sample
  • Flexible – enables reliable characterization of a broad range of sequence differences
  • Fast – rapid PCR amplification prior to high resolution melting, enabling higher throughput

Product Description

SensiFAST HRM Kit has been developed for detailed characterization of samples according to their base composition, length and GC content by high resolution melting. The latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensure the SensiFAST HRM Kit delivers reproducible, accurate HRM analysis.

SensiFAST HRM Kit contains EvaGreen®, a third generation, saturating fluorescent dye which selectively binds to double-stranded DNA. In contrast to dyes such as SYBR® Green I, EvaGreen can be used at higher concentrations without inhibiting PCR and shows equal binding affinity for GC-rich and AT-rich regions. The combination of our SensiFAST DNA Polymerase, unique buffer system and EvaGreen dye enables amplification and discrimination of even the most challenging sequence differences, such as class IV SNPs without sequence preference. Since it does not require expensive labelled oligonucleotide probes, SensiFAST HRM Kit is also a cost effective alternative to traditional probe based genotyping methods.

Applications

  • SNP genotyping
  • Characterization of haplotype blocks
  • DNA mapping
  • Detection of insertions, deletions and translocations
  • DNA fingerprinting
  • DNA methylation analysis
  • Gene scanning
  • Species identification
  • Allelic prevalence population studies
  • Screening for loss of heterozygosity
Main

Introduction to SensiFAST

Overview, features and benefits of the SensiFAST product family

One-step Vs. Two-step real-time RT PCR

A discussion of the pros and cons of each detection strategy.

We used the Bioline SensiFAST HRM mix to discriminate changes at a locus with substitutions on both strands, yielding AT heteroduplexes as well as either A or T monoduplexes. We easily achieved calls on the mono duplexes over heteroduplexes with more than 99% confidence to provide extremely sensitive genotyping of an AT class 4 SNP.

Dr Simon Baker, Senior Director of R&D, Bioline, London

Instrument Compatibility

Please refer to the Real-Time PCR Selection Chart to confirm the SensiFAST™ HRM Kit is compatible with your instrument.


Specification

Components

Reagent

500 x 20 µL reactions

2000 x 20 µL reactions

SensiFAST HRM mix (2x)

5 x 1 mL

20 x 1 mL

Volume

IO-32005: 500 x 20 µL Reactions: 5 x 1 mL
BIO-32020: 2000 x 20 µL Reactions: 20 x 1 mL

Storage & Stability

All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label. Avoid exposure of the EvaGreen to light.

Shipping conditions

SensiFAST HRM Kit is shipped on dry/blue ice.