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ISOLATE II PCR and Gel Kit is the simplest option for the purification of PCR products and for the isolation of DNA fragments from TAE and TBE agarose gel slices. A fast and easy-to-follow protocol is given for each application.
PCR products can be purified in 10 minutes using simple binding and elution steps. Concentrated PCR products ranging between 60 bp and 15 kb can be eluted, removing primers, nucleotides, enzymes, mineral oil, salts and other impurities.
DNA fragments between 50 bp and 20 kb can be extracted from agarose gel slices in 20 minutes using a color indicator to help maintain optimal pH and identify undissolved agarose.
ISOLATE II PCR and Gel Kit has been designed to deliver optimal performance in downstream applications, including transformations, cloning, sequencing and restriction analysis.
|
Reagent |
10 Preps |
50 Preps |
250 Preps |
|
ISOLATE II PCR and Gel Columns (yellow) |
10 |
50 |
250 |
|
Collection Tubes |
10 |
50 |
250 |
|
Binding Buffer CB |
10 mL |
40 mL |
200 mL |
|
Wash Buffer CW (Concentrate) |
6 mL |
25 mL |
2 x 50 mL |
|
Elution Buffer C |
13 mL |
13 mL |
30 mL |
|
Bench Protocol Sheet |
1 |
1 |
1 |
All components should be stored dry and at room temperature.
When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.
Shipped at ambient temperature.
Yes, though this may require some optimization. Warming the elution buffer to 50°C or even 70°C before applying it to the column can help to elute long PCR products, but there is the possibility using this adaptation to the protocol that the primers will be eluted too.
For the optimal recovery of large RNA/DNA fragments it is necessary to optimize the elution step. To increase the recovery rate it would be helpful to use a larger volume for elution, incubate the column with the elution buffer prior to centrifugation and use repeated elution steps. To avoid excessive dilution it may be helpful to reapply the eluted fraction again. Furthermore it could be helpful for DNA elution to heat the elution buffer to 70°C.
