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Next Generation SequencingFast qPCR & 2-Step RT-qPCR
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DNA Library Preparation
DNA Library Quantification
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To view our range of antigens and antibodies for immunoassay development please visit our partner site meridianlifescience.com
PCR-Amplified Libraries
Bringing high sensitivity, speed and efficiency to next-generation sequencing (NGS).
The JetSeq Flex DNA Library Preparation Kit contains all of the enzymes and buffers necessary for end repair, A-tailing and ligation in a convenient, optimized formulation. JetSeq Flex also includes the polymerase and buffer components required for PCR amplification of the library prior to sequencing. JetSeq Flex is optimized for use with sequence adapters compatible with Illumina® instruments, allowing indexing of up to 96 samples.
- Flexible – optimized for use with Illumina-compatible sequencing adapters for indexing up to 96 samples
- Low input – end repair, A-tailing and ligation combined in the same tube, thereby eliminating clean up steps and minimizing sample loss
- Improved confidence – simpler protocol with fewer steps for reduced risk of sample loss and offering greater peace-of-mind
- Increased speed – library preparation in less than 155 minutes for reduced time to results and increased throughput
- Highly efficient – reaction buffer pre-optimized to provide maximum end-repair, A-tailing and ligation efficiency
- High-yield – PCR polymerase and buffer developed specifically for library preparation, giving maximum yield of sequence-ready library DNA
- Improved quality – reliable library preparation from even very challenging samples, proving maximum coverage
Performance Data
Introduction; JetSeq™ DNA Library Preparation Kit
NGS Selection Chart
Select the best reagent for your research based on your library preparation requirements.PCR-Free Libraries
The JetSeq ER & Ligation Kit contains all of the enzymes and buffers necessary for end repair, A-tailing and ligation in a convenient, optimized master mix formulation. This kit is recommended for NGS applications that do not require PCR amplification of the library prior to sequencing. JetSeq ER & Ligation Kit is optimized for use with sequence adapters compatible with Illumina® instruments, allowing indexing of up to 96 samples.
- Flexible – optimized for use with Illumina-compatible sequencing adapters for indexing up to 96 samples
- Low input – end repair, A-tailing and ligation combined in the same tube, thereby eliminating clean up steps and minimizing sample loss
- Improved confidence – simpler protocol with fewer steps for reduced risk of sample loss and offering greater peace-of-mind
- Increased speed – library preparation in less than 60 minutes for reduced time to results and increased throughput
- Highly efficient – reaction buffer pre-optimized to provide maximum reaction efficiency and conversion rates
- Improved quality – reliable library preparation from even very challenging samples, proving maximum coverage
Performance Data
Introduction; JetSeq™ DNA Library Preparation Kit
Library Quantification
Accurate quantification of the number of amplifiable library molecules loading onto the flow cell is a critical step in the NGS workflow in obtaining high-quality read data. The JetSeq Library Quantification Kit provides all of the components, including JetSeq FAST SYBR® mix, primers and DNA standards of known concentration to allow quantification of library DNA.
- Accurate - qPCR-based assay for quantification of only adapter-ligated library molecules, thereby enabling optimal flow cell loading for maximum data yield and quality.
- Sensitive - reliable quantification of even low-yield libraries, ideal for both PCR and PCR-free library preparation methods
- Fast - delivers accurate assay results in as little as 90 minutes, thereby reducing time to results.
- Convenient - contains a series of six pre-diluted DNA standards for rapid, simple standard curve development.
- Economical - contains sufficient reagents and standards to quantify eighteen individual libraries on separate plates.
Performance Data
Introduction; JetSeq™ DNA Library Preparation Kit
Purification & Size Selection
JetSeq Clean is based on paramagnetic bead technology, designed for efficient purification of nucleic acid fragments as well as a single- or double-sided size selection of libraries following the fragmentation, ligation and PCR steps in the next-generation sequencing (NGS) library preparation workflow.
- Precise - highly-reproducible selection of the user-defined fragment range
- Efficient - excellent recovery of fragments and efficient removal of all contaminants
- Flexible - highly effective clean-up from all types of fragmentation, ligation and PCR reactions
- Fast - straightforward integration with automated liquid handling platforms
- Robust - developed to withstand the rigours of the NGS workflow