- Research Areas
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Next Generation SequencingFast qPCR & 2-Step RT-qPCR
Fast 1-Step RT-qPCR
Standard qPCR & 2-Step RT-qPCR
Real-Time PCR Genotyping
qPCR & RT-qPCR Extraction Controls
cDNA Synthesis
DNA Library Preparation
DNA Library Quantification
Purification & Size Selection
End-Point PCR
Standard PCR
Robust PCR
High-Fidelity PCR
Fast PCR
Long PCR
RT-PCR
Direct PCR
Reverse Transcription
Molecular Biology Reagents
Electrophoresis
Cloning
Sample PreparationFirst Generation Polymerases & Mixes
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To view our range of antigens and antibodies for immunoassay development please visit our partner site meridianlifescience.com
DNA Library Preparation
JetSeq™ Lyophilized NGS Library Prep Kits are designed to be a straightforward sustainable workflow, just add the fragmented DNA, then the adapters. They are ready-to-use, consisting of an end repair/dA-tailing mix module to convert fragmented DNA into 5’-phosphorylated and 3’-dA-tailed DNA fragments, a ligase module, for the addition of sequencing adapters and an optional high-fidelity PCR module for library amplification.
- Lyophilized reactions in sealed pouches for ambient temperature stability, transport and storage
- Ready-to-use, just add DNA
- Cost-effective, sustainable and accessible testing for NGS
Library Quantification
Accurate quantification of the number of amplifiable library molecules loading onto the flow cell is a critical step in the NGS workflow in obtaining high-quality read data. The JetSeq Library Quantification Kit provides all of the components, including JetSeq FAST SYBR® mix, primers and DNA standards of known concentration to allow quantification of library DNA.
- Accurate - qPCR-based assay for quantification of only adapter-ligated library molecules, thereby enabling optimal flow cell loading for maximum data yield and quality.
- Sensitive - reliable quantification of even low-yield libraries, ideal for both PCR and PCR-free library preparation methods
- Fast - delivers accurate assay results in as little as 90 minutes, thereby reducing time to results.
- Convenient - contains a series of six pre-diluted DNA standards for rapid, simple standard curve development.
- Economical - contains sufficient reagents and standards to quantify eighteen individual libraries on separate plates.
Performance Data
Purification & Size Selection
JetSeq Clean is based on paramagnetic bead technology, designed for efficient purification of nucleic acid fragments as well as a single- or double-sided size selection of libraries following the fragmentation, ligation and PCR steps in the next-generation sequencing (NGS) library preparation workflow.
- Precise - highly-reproducible selection of the user-defined fragment range
- Efficient - excellent recovery of fragments and efficient removal of all contaminants
- Flexible - highly effective clean-up from all types of fragmentation, ligation and PCR reactions
- Fast - straightforward integration with automated liquid handling platforms
- Robust - developed to withstand the rigours of the NGS workflow