SensiFAST™ cDNA Synthesis Kit

• "My aim is to isolate as much RNA as possible from a small amount of primary cells, which then are used for further qPCR experiments.
  With the help of Bioline products (ISOLATE II RNA Micro Kit and SensiFAST cDNA Synthesis Kit) it is possible to achieve the best results with the lowest amount of sample."

  Ms. Barth, Medical Faculty of the University of Saarland, Germany

• I had been repeating an experiment over and over again because I wasnt getting any amplicon of my target gene after cDNA synthesis and qPCR, thinking my differentiation protocol wasnt working until I tried the SensiFAST cDNA Synthesis Kit and bammmmm, the amplicon was there. I was using more than 50 ng/µl with a different company and wasn't getting any result but with just 10 ng/µl on the SensiFAST cDNA Synthesis Kit, I get to save some RNA for future reference and get the data I am looking for. Are you working with lesser concentration of RNA that you need to be able to replicate your experiments? SensiFAST cDNA Kit is for you then!

  Dad Abu-Bonsrah, Royal Children's Hospital (MCRI) - Embryology Department, Australia

• Working with post-mortem human heart tissue samples for a gene expression project proved to be a challenging task for tissue samples that came from the medical examiner's office. So many factors were working against a viable gene expression profile for these samples such as time of death to autopsy lag, time from autopsy to tissue sample, and whether or not the tissue had been flash frozen. The samples were also never treated with RNA-later so we expected highly degraded RNA from these tissue samples.
  
  Our only light of hope was the fact that our target amplicon sizes were less than 100 bp which meant that we could still find what we were looking for in spite of highly degraded RNA. Chances were that at least a few intact sequences would amplify. The critical part in our experiment was to be able to yield enough cDNA from whatever bit of RNA we were able to extract from our samples.
  
  This is where Bioline exceeded all expectations. Working quickly and carefully, I was able to get decent cDNA considering all the factors working against my RNA isolations. The cDNA was enough to be able to carry out a gene expression profile for each of the samples and our project was a success. Bioline proved to be superior in cDNA yield from highly degraded RNA samples.

  Daniel Perez, Heart Institute, Los Angeles, US

• I have been using Bioline products for past 5 years, starting with MyTaqRed Mix and dNTPs. Recently, we started using the SensiFAST cDNA Synthesis Kit and SensiFAST SYBR mix for qRT-PCR. Upon testing with diverse RNA sources, we were really satisfied with the results as the data is quite comparable with competitor SYBR mixes. Quality to price ratio is the winning factor for both the cDNA synthesis kit and SYBR mix. We felt real ease-of-use with the cDNA synthesis kit, which was fast and simple. I recommend the SensiFAST cDNA Synthesis kit and SensiFAST SYBR mix from Bioline.

  Lakshmi Palam, Indiana University School of Medicine, Indianapolis, US

• The SensiFast cDNA Synthesis Kit was directly compared to another cDNA synthesis kit. During real-time qPCR, the CT values of the cDNA samples produced with the SensiFast cDNA synthesis Kit lay between 16 and 18. The samples behaved very consistent and results were highly reproducible. The cDNA samples produced with the other kit had CT values between 24 and 28 and were much more scattered.

  Anna Kipp, German Institute of Human Nutrition, Nuthetal, Germany

• We used the Isolate II RNA Micro Kit, SensiFAST cDNA synthesis kit and SensiFAST SYBR No-ROX Kit to perform our PCR and got excellent results. Our standard curves were great with r2 values over 0.99. Very impressed and pleased with this result, especially given it was our first time doing real time PCR. Hope you like the video review we put together!

  Daisy Shu, Bosch Institute, Anatomy and Histology, Camperdown, Australia

• We used the Isolate II RNA Micro Kit, SensiFAST cDNA synthesis kit and SensiFAST SYBR No-ROX Kit to perform our PCR and got excellent results. Our standard curves were great with r2 values over 0.99. Very impressed and pleased with this result, especially given it was our first time doing real time PCR. Hope you like the video review we put together!

  Daisy Shu, Bosch Institute, Anatomy and Histology, Camperdown, Australia

• Our Institute consisting of more than 60 active researchers has a longstanding interest in understanding changes to the transcriptional landscape of prostate cancer. Reproducibility, sensitivity and cost-effectiveness are critical parameters in selecting our day-to-day cDNA synthesis kit. This combined with its convenient two reagent system continues to make the SensiFast cDNA synthesis kit stand out from its competitors and our preference for almost two years.

  Martin Sadowski, Queensland University of Technology, Brisbane, Australia

• After multiple years using products from other companies, we have now trialed and switched to using Bioline products for many aspects of our work. We use the Bioline RNA extraction kits, SensiFast cDNA synthesis and SYBR Lo-Rox kits for our transcriptional analysis and are very happy with the results this provides. The sensitivity is as good as if not better than other products, and is more cost beneficial as well. The rep we deal is always available to help us in any way we need, which helps makes our experience with Bioline simple and effective.

  Sean Wilson, Murdoch Childrens Research Institute, Parkville, Australia

• We recently trialled Bioline's SensiFAST cDNA Synthesis Kit and the SensiFAST Probe Lo-Rox Kit.
  
  Compared to other cDNA synthesis kits we have used, the SensiFAST product is both simple and efficient. All components are included in only two reagents; this onestep methodology limits both variability and preparation time. Furthermore the reaction speed is very fast, allowing us to generate high quality cDNA from low yield RNA samples in less than an hour.
  
  We then utilised the SensiFAST Probe Lo-Rox kit coupled with Taqman probes (Applied Biosystems) to prepare our cDNA samples for qPCR. Again, the kit was very simple and using the Viia-7 Real-Time PCR system (Applied Biosystems) we were able to produce fast and consistent results. As a result of our preliminary tests we are expanding our use of these reagents to in our quantitative assays.
  
  Overall we have found Bioline’s products to be of excellent quality and great value. Their staff have been very helpful and always willing to offer advice in a timely manner.
  

  Krista D, The Florey Institute, Parkville, Australia

• We have been using Bioline products (especially the Isolate II RNA mini Kit, cDNA synthesis Kit and Sensifast SBYR mix) for past 3 years, and we are very happy with the consistency of results that we obtained and also the good price they offered. We are happy to continue using these products.

  Kuan Won, Mater Research Institute, Brisbane, Australia

• Our lab uses Bioline Sensi FAST cDNA synthesis kit for various samples and it has proven to work efficiently each time. Consistent results were obtained. Since 5X master mix is already provided we need not add any primers or DTT so saves a lot of time in setting up reaction. I love the product.

  Sanskruti Lakhotia, RMIT University, Australia

• I recently trialed Bioline's SensiFAST cDNA Synthesis kit. The kit is very fast and easy to use, allowing cDNA to be generated from low yields of mRNA in under 1 hour. I have compared a standard housekeeping gene (Gapdh) by qPCR using Bioline's SensiFAST SYBR No-ROX kit using 1.25ng cDNA prepared from mouse liver mRNA using the SensiFAST cDNA Synthesis kit and another cDNA kit from a competitor. The Ct's obtained using the Bioline kit were consistently lower than that of the competitor. Overall, this is a great product and very easy to use, I will certainly be using this product in the future.

  Cavan Bennett, Walter and Eliza Hall Institue of Medical Research, Melbourne, Australia

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