EasyLadder I

All HyperLadders and EasyLadders are ready-to-load and require no addition of loading buffer prior to use. In general, the smaller the DNA product, the higher the percentage of Agarose required in the gel. As a guide for optimization we would suggest to use the EasyLadder I with 2% (w/v) agarose gel,

Hyperladder 100bp with 2% (w/v) agarose gel,

Hyperladder 1kb with 1% (w/v) agarose gel,

Hyperladder 50bp with 1.5% (w/v) agarose gel and

Hyperladder 25bp with 3.5% (w/v) agarose gel.

Please have a look into the related application note for more information about ladder migration in different gel concentrations.

Problems with our HyperLadder and EasyLadder are extremely rare, so please make sure you have carried out each of the following correctly:

-          Used the correct percentage of agarose in your gel for the specific marker in question.

-          Used 5 µL of the marker per lane.

-          Used the correct concentration of stain (e.g. EtBr) to visualise correctly.

-          Vortexed the marker gently before use.

Should problems persist, please contact technical services for further assistance.

No, our Ladder range is supplied in a ready-to-load format to be directly loaded onto your agarose gel. An additional vial of 5x Loading Buffer is supplied with each of our markers for use with your samples.

Other stains, such as SYBR^® Green Gel Stain, can be used with our ladders as effectively as EtBr. The fluorescence of the gel as a whole may vary depending on the stain used, however the relative intensities of the marker bands and of the samples run will remain the same, thus the HyperLadder / EasyLadder remains quantitative.

EasyLadder I: gel with 2% (w/v) agarose in TBE

HyperLadder 1kb: gel with 1% (w/v) agarose in TBE

HyperLadder 100bp: gel with 2% (w/v) agarose in TBE

HyperLadder 50bp: gel with 1,5% (w/v) agarose in TBE

HyperLadder 25bp: gel with 3,5% (w/v) agarose in TBE

For all 5 µL ladder have been applied and visualized using ethidium bromide.