Uracil DNA Glycosylase

Product Highlights

• Removes uracils from DNA - causes fragmentation of DNA and stops amplification in a PCR
• Easy-to-use - novel, sensitive mutant is irreversibly inactive after heating

Product Description

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).

UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM).

Meridian UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.

Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.

Applications

• Control of carry-over contamination in PCR
• As a probe for protein-DNA interaction studies
• Cloning of PCR products