Thermostable Reverse Transcriptase

Product Highlights

• Stable - Reverse transcriptase activity up to 60°C
• Robust - Ideal for RNA with high secondary structure such as viral genomes
• Sensitive - Detection of low copy number RNA targets
• Fast - Ideal for developing fast, highly reproducible RT-qPCR assays

Product Description

Traditional Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is not thermostable and can only maintain its enzymatic activity at relatively low temperatures (up to 50°C). However for cDNA synthesis, a higher reaction temperature is desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimizes nonspecific primer binding.

Meridian Thermostable Reverse Transcriptase has higher thermal stability and reduced RNase H activity. The enzyme can be used to synthesize first-strand cDNA at temperatures up to 60°C which improves the cDNA yield from difficult RNA targets that require higher temperature to denature strong secondary structures.