SensiFAST™ Probe has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, molecular beacon and Scorpions® probes. A combination of the latest advances in buffer chemistry and PCR enhancers, in conjunction with an antibody-mediated polymerase promote rapid, consistently accurate detection of DNA and RNA targets from a broad range of sample types.
Improved consistency between technical replicates
Reliable quantitation of low abundance targets
Reproducible, accurate results in as little as 30 minutes
Download the SensiFAST qPCR Guide with detailed product descriptions and performance data to help you choose the best product for your research
“I performed a comparison of Meridian's SensiFAST™ Probe against several other qPCR master mixes. The Meridian product performed the best overall in terms of efficiency, sensitivity and lower cost. I have also been very impressed with Meridian's customer service.”
Tiffany Kosch, James Cook University, Townsville, Australia
Probe One-Step
The SensiFAST™ Probe One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube and uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. This also gives SensiFAST Probe One-Step unbeatable efficiency in multiplexing. SensiFAST Probe One-Step Kits consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.
Novel 1-step buffer optimized for highly-efficient RT and PCR steps
Improved consistency between technical replicates
Reproducible, accurate results in as little as 40 minutes
Select the best reagent for your research based on your instrument and sample requirements
“Very good linearity right down to 10 copies, very good correlation coefficient, good qPCR reaction efficiency and gave a single band on an agarose gel.”
King’s College London, UK
SYBR®
The SensiFAST™ SYBR® Kit has been developed for fast, highly-sensitive and reproducible qPCR and has been validated on commonly-used real-time PCR instruments. The use of an antibody-mediated hot-start DNA polymerase minimizes amplification from primer-dimers, thereby improving assay specificity and sensitivity.
Improved consistency between technical replicates
Reliable quantitation of low abundance targets
Reproducible, accurate results in as little as 30 minutes
Download the SensiFAST qPCR Guide with detailed product descriptions and performance data to help you choose the best product for your research
SYBR® One-Step
SensiFAST™ SYBR® One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® One-Step Kits use a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. SensiFAST One-Step Kits consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.
Optimized 1-step buffer for highly-efficient reverse transcription and amplification
Improved consistency between technical replicates
Reproducible, accurate results in as little as 40 minutes
Select the best reagent for your research based on your instrument and sample requirements
“SensiFAST SYBR No-ROX Kit is a wonderful product, it makes all the difference between a successful and an unsuccessful experiment.”
Alcorn State University, USA
Genotyping
The SensiFAST™ Genotyping Kit has been developed for high-performance dual-labelled probe assays that deliver fast, precise and highly reproducible characterization of sequence variants, including loci with type IV SNPs. The optimized buffer system promotes clear allelic discrimination and outstanding allele clustering.
Clear discrimination of all alleles
Tight fluorescence clusters
Reproducible results in as little as 30 minutes
Reliable genotyping from even very limiting amounts of sample
Genotyping from a wide range of samples
SensiFAST™ HRM Kit is a post-PCR analysis method of characterizing nucleic acid samples based on their DNA strand dissociation behavior, with increasing temperature, in the presence of saturating fluorescent dyes. Previously unknown and even complex sequence variations can be easily detected.
Clear discrimination of even the most challenging sequence differences
Unparalleled consistency between replicate difference plots
Efficient detection from limiting amounts of sample
Reliable characterization of a broad range of sequence differences
Overview, features and benefits of the SensiFAST HRM Kit
“In the course of optimizing real-time PCR for analysis of various animal tissues, I tested multiple real-time master mixes of different brands. The SensiFAST™ SYBR No-ROX Kit produced the most valid amplification result by improving efficiency and reproducibility. With this product, Meridian offers an optimal cost-performance ratio.”
Department of Animal Nutrition, TU Munich, Germany
cDNA Synthesis
For 2-step RT-qPCR, Meridian has developed the SensiFAST™ cDNA Synthesis Kit to complement the SensiFAST Probe and SYBR® Kits. The unique combination of our high-performance reverse transcriptase and optimized TransAmp™ buffer system delivers excellent reverse transcription efficiency across a very broad range of input RNA concentrations.
Unique reverse transcriptase and TransAmp™ buffer generate consistent first-strand cDNA
Reliable analysis from as little as 1 pg of starting total RNA
Broad dynamic range enabling quantification of dilute and low-copy samples
Unbiased cDNA synthesis for complete 5’ to 3’ RNA sequence representation
Reverse transcription and qPCR complete in less than 60 minutes
Overview, features and benefits of the SensiFAST cDNA Synthesis Kit
“I have been using a Meridian qPCR mix (SensiFAST SYBR No-ROX, BIO-98020) for four years with Arabidopsis cDNA on a Bio-Rad CFX-96 and a Roche LightCycler-96 in 15 or 20 ul reaction volumes. I can highlight excellent technical replication, low baseline noise and good signal/noise ratio. Reasonable price quotations allow me also to stretch my budget. I know then that with an appropriate experimental design I can trust my results and expect them on time!”
R. Alvarez, University of Essex, UK
Reviews
"I have been using a Meridian's qPCR mix (SensiFAST SYBR No-ROX, BIO-98020) ..."
R. Alvarez, University of Essex, UK
"Very good linearity right down to 10 copies, very good correlation coeffic ..."
King's College London, UK
"Great product, worked exactly how expected, and sensitive to small amounts ..."
University of South Carolina, USA
"SensiFAST SYBR No-ROX Kit is a wonderful product, it makes all the differe ..."
Alcorn State University, USA
"In the course of optimizing real-time PCR for analysis of various animal t ..."
Department of Animal Nutrition, TU Munich, Germany