MyTaq Blood-PCR Kit offers very fast, highly-specific, direct PCR from a wide range of human and animal whole blood samples, including those preserved with anticoagulants.
MyTaq™ Blood-PCR Kit is recommended for fast, specific and direct PCR from human and animal blood samples. MyTaq Blood-PCR Kit incorporates MyTaq HS DNA Polymerase, the latest generation of very high-performance polymerases unique to Bioline. Furthermore, MyTaq HS has an increased affinity for template DNA, giving a high PCR product yield from the most challenging templates.
The combination of a unique, inhibitor-tolerant buffer system and MyTaq HS DNA Polymerase, ensures the MyTaq Blood-PCR Kit overcomes the PCR inhibitors typically present in blood samples, including anticoagulants (EDTA, citrate and heparin). This leads to significantly increased sensitivity and PCR success rates even with demanding applications such as long amplicons and GC-rich templates In addition to supporting robust PCR amplification, the novel buffer system replaces the need for complicated extraction and purification steps or the use of additives.
The speed and high specificity of MyTaq Blood-PCR Kit makes it highly suited for multiplex PCR and high-throughput genotyping assays. The advanced formulation of MyTaq Blood-PCR Kit allows fast cycling conditions to be used, without compromising PCR specificity and yield.
MyTaq Blood-PCR Mix, 2x
5 x 625 µL
MyTaq Blood-PCR Kit can be stored at -20°C. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label.
On dry or blue ice.
|No or low PCR yield||Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.|
|Primers degraded – check quality and age of the primers.|
|Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM.|
|Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration.|
|Template concentration too low – Increase concentration of template.|
|Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.|
|Multiple Bands||Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.|
|Prepare master mixes on ice or use a heat-activated polymerase.|
|For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.|
|Smearing or artifacts||Template concentration too high. Prepare serial dilutions of template.|
|Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.|
|Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.|
|Extension time too long. Reduce extension time in 0.5-1 minute increments.|