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MangoMix™

Ordering

Cat. No.
Size
BIO-25033
250 x 50µl Reactions
BIO-25034
1000 x 50µl Reactions

*link will take you to our exclusive distribution partner site

Description

MangoMix™ is a convenient, ready-to-go, 2x Reaction Mix containing MangoTaq™ DNA Polymerase, MgCl2 and ultra-pure dNTPs manufactured by Meridian. The Mix is optimized and ready-to-use and requires only the addition of water, template and primers.

Product Highlights

  • Direct gel loading - no need for further post-PCR processing steps
  • Easy visual recognition- reduces pipetting errors
  • High performance - pre-optimized 2x solutions
  • Ready to use format - reduces risk of contamination and decreases the reaction set-up time
  • Reproducible results - consistent QC ensures reliability

Product Description

MangoMix™ is optimized and ready-to-use, so the user need only add water, template and primers. MangoMix reduces the time required to set up reactions, thereby minimizing contamination risks and providing greater reproducibility through a reduction in the number of pipetting steps. MangoMix can be loaded directly onto an agarose gel for analysis, without the need for a separate gel-loading buffer.

The presence of dyes has no effect on routine enzymatic manipulations, although rare exceptions may exist. MangoMix has been optimized for a wide variety of templates. An additional 50 mM of MgCl2 solution is included should any fine adjustments be required.

Applications

  • High throughput applications
  • Suited to a wide range of PCR assays
  • Products suitable for TA cloning
  • Direct loading
Main

PCR Enzyme Guide

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PCR Selection Chart

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Specification

Components

Reagent

250 Reactions

1000 Reactions

MangoMix

5 x 1.25 mL

20 x 1.25 mL

50 mM MgCl2 Solution

1.2 mL

1.2 mL

Concentration

2x

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

On Dry Ice or Blue Ice.



Resources

Documents

MangoMix - Product Manual
MangoMix - Fact Sheet
MangoMix - MSDS

Latest Certification of Analysis (COAs)

BIO-25033_PM335-B126430

FAQs

When using MangoMix, is there a need to use loading buffer when loading PCR samples onto a gel?

No, the dyes and composition of MangoMix are such that the samples will sink easily into the well and the samples can be clearly seen, thus no loading buffer is required to load your samples on an agarose gel.

Will the dyes present in MangoMix interfere with further post-PCR experiments?

The two colored dyes present in this mix are completely inert and thus will have no effect on downstream applications. An exception is the quantification of PCR products in colored buffers with photometric methods or fluorescence assays. We cannot exclude the impact of these dyes on quantification results and suggest the purification of these samples. If you are concerned about these dyes interfering in your specific applications, we would recommend the cleanup of your samples using the ISOLATE II PCR & Gel Kit or SureClean Plus.

What is the final working concentration (at 1x) of MgCl2 in each mix?

Meridian's mixes contain magnesium chloride at the following concentrations (please see table below). An additional tube of 50 mM MgCl2 solution is supplied with each mix to facilitate further optimization if required.



 Meridian Mix Final Magnesium Concentration
ACCUZYME Mix  2.0 mM.
BioMix / BioMix Red  2.5 mM.
ImmoMix / ImmoMix Red  3.0 mM.
BIO-X-ACT Short Mix  2.0 mM.
MangoMix  2.5 mM.
MyTaq  3.0 mM.
RANGER  1.5 mM.



Which polymerase do I need for my specific application?

At Meridian we pride ourselves in supplying high-quality polymerases to suit your requirements. To aid your selection of the most suited enzyme for your specific applications, please see our enzyme selection tool.

What are the storage conditions and stabilities of Meridian polymerases?

All our polymerases are guaranteed for a period of 12 months from the date of purchase. These should be stored at -20°C during this time for optimal retention of activity.

Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.

I am having problems optimizing my PCR, what would you recommend?

PCR can be a challenging technique, with various parameters to optimize to achieve the best results. If you are having problems, these could be easily resolved by addressing a few issues. Please see our PCR troubleshooting guide for suggestions and help with your specific problems.

Observation Recommended Solution(s)
No or low PCR yield  Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.
 Primers degraded – check quality and age of the primers.
 Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting  concentration of 1.75 mM.
 Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both  primers have the same concentration.
 Template concentration too low – Increase concentration of template.
 Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.
Multiple Bands  Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.
 Prepare master mixes on ice or use a heat-activated polymerase.
 For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.
Smearing or artifacts  Template concentration too high. Prepare serial dilutions of template.
 Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.
 Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.
 Extension time too long. Reduce extension time in 0.5-1 minute increments.


What do the terms yield, fidelity, processivity and specificity actually mean?

These terms refer to parameters to be considered when performing PCR and are important features in selecting the correct enzyme for your needs. Understanding what they mean is therefore crucial: Yield: The amount of DNA produced in a PCR reaction. Fidelity: The accuracy of the enzyme at incorporating the correct dNTP to the elongating DNA strand. Processivity: The length of time a polymerase is associated with the template and therefore the size of fragment which can be amplified. Specificity: A measure of the unwanted by-products generated in a reaction.

How is one unit of activity of a polymerase defined?

One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C.

What are the advantages of using a 2x mix rather than setting up a PCR reaction from scratch?

All Meridian polymerases are available in the convenient format of a 2x master mix, containing all the reagents and additives necessary to perform successful PCR, with the exception of template, primers and water. This formulation not only provides a convenient and hassle-free PCR setup, but also significantly reduces the chances of human error, inaccuracies and contamination by reducing the pipetting steps required.

What is the recommended reaction volume of the polymerase mixes?

We recommend a final reaction volume of 50 µL, this requiring the use of 25 µL of the 2x master mix and to make up to 50 µL using template, primers and PCR grade water.

What is the composition of the polymerase mixes?

Whilst the exact composition of the mixes is proprietary information, all mixes are made up of reaction buffer, magnesium, dNTPs, polymerase and additives, designed to keep the polymerase stable in the mix, as well as provide the optimal conditions for PCR.

What is the concentration of the polymerase mixes?

All Meridian PCR mixes come supplied at a 2x concentration, requiring the user simply to add template, primers and PCR grade water to a final concentration of 1x.

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