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Ordering

Cat. No.
Size
BIO-52065
10 Preps
BIO-52066
50 Preps
BIO-52067
250 Preps

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Description

ISOLATE II Genomic DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of materials, without the need for hazardous reagents such as phenol

Product Highlights

  • Fast – streamlined protocol for consistent recovery of genomic DNA in as little as 20 minutes
  • High-performance – extraction of high-purity DNA, ideal for use in all downstream applications
  • High-yield – efficient sample lysis and column-binding for high-yield recovery of genomic DNA from limiting amounts of sample
  • Flexible – reliable extraction of genomic DNA from a very broad range of human, animal and microbial samples

Product Description

By combining Proteinase K lysis with the speed and convenience of silica membrane purification, ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials.

By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded issue (FFPE), insects, dental swabs, buccal swabs, stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgordferi) and viral DNA (e.g. CMV) from urine.

ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with SensiFAST Real-Time PCR Kits and in end-point PCR with MyTaq DNA Polymerase.

Applications

  • qPCR
  • End-point PCR
  • Southern, dot and slot blotting
  • Genotyping
  • Restriction digestion
  • Next Generation Sequencing
  • Bisulfite conversion/methylation analysis
Main

Nucleic Acid Isolation Guide

Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your research

I used the ISOLATE II Genomic DNA Kit to extract genomic DNA from mice tails for genotyping. The kit comes with very clear, user friendly instructions and provides efficient, reliable and cost effective isolation of DNA. Overall a great kit for genomic DNA extraction and downstream analysis.

Wilson Wong, University of Sydney, Camperdown, Australia

Specification

Components

Reagent

10 Preps

50 Preps

250 Preps

ISOLATE II Genomic DNA Spin Columns

10

50

250

Collection Tubes (2 mL)

20

100

500 

Lysis Buffer GL

5 mL

20 mL

100 mL

Buffer G3

10 mL

15 mL

75 mL

Wash Buffer GW1

6 mL

30 mL

150 mL

Wash Buffer GW2 (concentrate)

6 mL

12 mL

50 mL

Elution Buffer G

13 mL

13 mL

60 mL

Proteinase K (lyophilized)

6 mg

30 mg

2 x 75 mg

Proteinase K Buffer PR

1.8 mL

1.8 mL

8 mL

Bench Protocol Sheet

1

1

1

Storage & Stability

All components should be stored dry and at room temperature.

When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

Ambient temperature.



Resources

Reviews

"I recently trailed the ISOLATE II Genomic DNA kit to isolate gDNA from ... "

"I have used this kit to extract genomic DNA from mice tails (approx. 0.6 cm) ... "

"The ISOLATE II Genomic DNA Kit is extremely sensitive. It produced ... "

FAQs

The columns supplied with the ISOLATE II kits may appear similar, but each type has been optimized to work within the buffer system supplied with the corresponding kit. The swapping of columns (or buffers) between kits may lead to no recovery of nucleic acid whatsoever, or at the very least severely impaired purification.

Interruption of the DNA/RNA extraction process is possible after the sample lysis only. It is possible to homogenize and lyse the samples and to store them in the freezer until use for RNA extraction. RNA clean up with a column cannot be interrupted and we recommend to avoid delays during the column purification process. If a delay is unavoidable the columns should be stored on ice.

The elution buffer is ideal for applications such as restriction enzyme digestion, sequencing and PCR. It is possible to use DNase-free water for elution, but you should expect a slightly lower yield.

The elution buffer of this kit does not contain EDTA which could interfere with downstream applications like PCR or sequencing.

The ISOLATE II Genomic DNA Kit provides a protocol for hard to lyse bacteria (see sec 9.2). Also the ISOLATE II Plant DNA kit could be suitable, because the optimized lysis buffer from this kits may be also be used for processing these difficult to lyse bacteria. In general, it is necessary to optimize the lysis step with the appropriate mechanical, enzymatic or chemical methods recommended for this kind of sample. Please refer to the related literature or ask the Bioline technical support for further suggestion.

For the optimal recovery of large RNA/DNA fragments it is necessary to optimize the elution step. To increase the recovery rate it would be helpful to use a larger volume for elution, incubate the column with the elution buffer prior to centrifugation and use repeated elution steps. To avoid excessive dilution it may be helpful to reapply the eluted fraction again. Furthermore it could be helpful for DNA elution to heat the elution buffer to 70°C.