Ordering
Description
Product Highlights
- Reduced recombination of cloned DNA - accommodates larger plasmids
- High efficiency - available in ≥108 cfu/μg, perfect for difficult clones
- Easy screening - with blue/white color selection
- Convenient size - small aliquots reduce waste
Product Description
α-Select Silver Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for difficult clone construction and blunt end ligation using plasmid-derived vectors.
α-Select Silver Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.
pUC19 DNA is also provided as a positive control.
Applications
- Transformation of large plasmids into bacterial cells
- Generating clones
- Blue/white color screening
Resources
Documents
Certification of Analysis (COAs)
Specification
Components
|
Reagent |
BIO-85026 |
BIO-85046 |
|
Chemically Competent Cells |
10 x 200µl |
20 x 50µl |
|
pUC19 control vector (10pg/µl) |
10µl |
10µl |
Storage & Stability
All components should be stored at -80°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
Shipping conditions
Shipped on dry ice
