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Description
Product Highlights
- Sensitive – exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
- Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
- Specific – an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
- Flexible – ideal for amplifying any target up to 5 kb, including DNA extracted from human, animal and plant samples
- Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
- Convenient – includes all the components necessary for high performance PCR amplification
Product Description
MyTaq™ HS DNA Polymerase is a new generation of antibody-mediated hot-start enzyme, engineered for highly specific and efficient amplification from even the most challenging templates. MyTaq HS remains inactive at room temperature allowing for convenient reaction set-up, thereby reducing non-specific amplification that can hinder PCR assays from the start. These properties make MyTaq HS DNA Polymerase the ideal choice for PCR assays containing complex and low copy number targets.
The inclusion of dNTPs, MgCl2 and enhancers at optimal concentrations in the buffer helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats. The optimized buffer system and MyTaq HS with its increased affinity for DNA, enables very high yield PCR amplification over a wide range of PCR templates, resulting in reliable amplification from even very low amounts of template.
The advanced formulation of MyTaq HS DNA Polymerase and buffer system has been developed to give more robust amplification than other commonly-used polymerases, meaning it performs reliably even in the presence of PCR inhibitors. Furthermore, it allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield.
Applications
- Fast PCR
- Multiplex PCR
- Genotyping
- Complex templates (e.g. GC-rich)
- Low copy number PCR assays
- High-throughput assays with prolonged PCR set-up
Introduction to MyTaq
Overview, features and benefits of the MyTaq product family
PCR Enzyme Guide
Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your researchPCR Selection Chart
Select the best reagent for your researchApplication Note
MyTaq™ HS DNA PolymeraseMyTaq HS DNA Polymerase is more robust than the other enzymes I’ve tried.
Sydney University, Save Sight Institute, Sydney, Australia
Product Selection
Please refer to the PCR Selection Chart to confirm the recommended product for your PCR application.Resources
Documents
Certification of Analysis (COAs)
Specification
Components
|
Reagent |
250 Units |
1000 Units |
2500 Units |
|
MyTaq HS DNA Polymerase |
1 x 50 µL |
1 x 200 µL |
2 x 250 µL |
|
5x MyTaq Reaction Buffer |
2 x 1 mL |
8 x 1 mL |
14 x 1.5 mL |
Concentration
5 u/µL
Storage & Stability
All components are shipped on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.
Components may also be stored at +4°C if required, although storage at -20°C is recommended. When stored at +4°C, reagents will remain stable for a period of 2 weeks from date of receipt.
Shipping conditions
On Dry Ice or Blue Ice.
FAQs
My DNA sample contains PCR inhibitors, which impairs my PCR results. What can I do for optimization?
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
| Observation | Recommended Solution(s) |
| No or low PCR yield | Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments. |
| Primers degraded – check quality and age of the primers. | |
| Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM. | |
| Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration. | |
| Template concentration too low – Increase concentration of template. | |
| Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated. | |
| Multiple Bands | Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers. |
| Prepare master mixes on ice or use a heat-activated polymerase. | |
| For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity. | |
| Smearing or artifacts | Template concentration too high. Prepare serial dilutions of template. |
| Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands. | |
| Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments. | |
| Extension time too long. Reduce extension time in 0.5-1 minute increments. |
Please click here in order to request your sample. You will receive an email confirmation within two business days with delivery details.
