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  • PCR-Amplified Libraries

    Bringing high sensitivity, speed and efficiency to next generation sequencing (NGS).

    The JetSeq Flex DNA Library Preparation Kit contains all of the enzymes and buffers necessary for end repair, A-tailing and ligation in a convenient, optimized formulation. JetSeq Flex also includes the polymerase and buffer components required for PCR amplification of the library prior to sequencing. JetSeq Flex is optimized for use with sequence adapters compatible with Illumina® instruments, allowing indexing of up to 96 samples.

    • Flexible – optimized for use with Illumina-compatible sequencing adapters for indexing up to 96 samples
    • Low input – end repair, A-tailing and ligation combined in the same tube, thereby eliminating clean up steps and minimizing sample loss
    • Improved confidence – simpler protocol with fewer steps for reduced risk of sample loss and offering greater peace-of-mind
    • Increased speed – library preparation in less than 155 minutes for reduced time to results and increased throughput
    • Highly efficient – reaction buffer pre-optimized to provide maximum end-repair, A-tailing and ligation efficiency
    • High-yield – PCR polymerase and buffer developed specifically for library preparation, giving maximum yield of sequence-ready library DNA
    • Improved quality – reliable library preparation from even very challenging samples, proving maximum coverage

    Performance Data

    <p><strong>Fig. 1 JetSeq Library Preparation workflow</strong></p>
             <p>The fast, one-tube JetSeq Library Preparation protocol offers the fastest turnaround time, with minimal cleanup steps reducing hands-on time and significantly reducing the total time required for preparation of library DNA.</p>

    Introduction; JetSeq™ DNA Library Preparation Kit

    PCR-Free Libraries

    The JetSeq ER & Ligation Kit contains all of the enzymes and buffers necessary for end repair, A-tailing and ligation in a convenient, optimized master mix formulation. This kit is recommended for NGS applications that do not require PCR amplification of the library prior to sequencing. JetSeq ER & Ligation Kit is optimized for use with sequence adapters compatible with Illumina® instruments, allowing indexing of up to 96 samples.

    • Flexible – optimized for use with Illumina-compatible sequencing adapters for indexing up to 96 samples
    • Low input – end repair, A-tailing and ligation combined in the same tube, thereby eliminating clean up steps and minimizing sample loss
    • Improved confidence – simpler protocol with fewer steps for reduced risk of sample loss and offering greater peace-of-mind
    • Increased speed – library preparation in less than 60 minutes for reduced time to results and increased throughput
    • Highly efficient – reaction buffer pre-optimized to provide maximum reaction efficiency and conversion rates
    • Improved quality – reliable library preparation from even very challenging samples, proving maximum coverage

    Products

    JetSeq ER & Ligation Kit

    Performance Data

    <p><strong>Fig. 1 JetSeq Library Preparation workflow</strong></p>
             <p>The fast, one-tube JetSeq Library Preparation protocol offers the fastest turnaround time, with minimal cleanup steps reducing hands-on time and significantly reducing the total time required for preparation of library DNA.</p>

    Introduction; JetSeq™ DNA Library Preparation Kit

    Library + Indexing

    The JetSeq DNA Library Preparation Kit contains all of the enzymes and buffers necessary for end repair, A-tailing, ligation and amplification in convenient, optimized formulations, as well as 16 barcoded adapters that can be used for single or multiplex reads.

    • Low input – end repair, A-tailing and ligation combined in the same tube, thereby eliminating clean up steps and improving yield
    • Improved confidence – simpler protocol with fewer steps for reduced risk of sample loss and offering greater peace-of-mind
    • Increased speed – library preparation in less than 3 hours for reduced time to results and increased throughput
    • Highly efficient – reaction buffer pre-optimized to provide maximum reaction efficiency and conversion rates
    • Improved quality – reliable library preparation from even very challenging samples, proving maximum coverage
    • Maximum convenience – sequencing adapters, enabling indexing of up to 16 samples, already included in the kit

    Performance Data

    <p><strong>Fig. 1 JetSeq Library Preparation workflow</strong></p>
             <p>The fast, one-tube JetSeq Library Preparation protocol offers the fastest turnaround time, with minimal cleanup steps reducing hands-on time and significantly reducing the total time required for preparation of library DNA.</p>

    Introduction; JetSeq™ DNA Library Preparation Kit

    Library Quantification

    Accurate quantification of the number of amplifiable library molecules loading onto the flow cell is a critical step in the NGS workflow in obtaining high-quality read data. The JetSeq Library Quantification Kit provides all of the components, including JetSeq FAST SYBR® mix, primers and DNA standards of known concentration to allow quantification of library DNA.

    • Accurate - qPCR-based assay for quantification of only adapter-ligated library molecules, thereby enabling optimal flow cell loading for maximum data yield and quality.
    • Sensitive - reliable quantification of even low-yield libraries, ideal for both PCR and PCR-free library preparation methods
    • Fast - delivers accurate assay results in as little as 90 minutes, thereby reducing time to results.
    • Convenient - contains a series of six pre-diluted DNA standards for rapid, simple standard curve development.
    • Economical - contains sufficient reagents and standards to quantify eighteen individual libraries on separate plates.

    Performance Data

    <p><strong>Fig. 1 JetSeq Library Preparation workflow</strong></p>
             <p>The fast, one-tube JetSeq Library Preparation protocol offers the fastest turnaround time, with minimal cleanup steps reducing hands-on time and significantly reducing the total time required for preparation of library DNA.</p>

    Introduction; JetSeq™ DNA Library Preparation Kit