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Description
Product Highlights
- Robust - enzyme blend and buffering system promotes reliable amplification of the most challenging and complex targets, even in the presence of inhibitors
- Sensitive - improved target affinity and high processivity ensure successful amplification in low-copy number assays
- Efficient - high-yield amplification of a broad range of targets up to 10 kb from human, animal and plant template DNA
- Specific - MyFi DNA Polymerase is an antibody-mediated hot-start blend that remains completely inactive during PCR set-up to prevent non-specific amplification
- Convenient - an all-in-one-tube mastermix that improves the speed, convenience and accuracy of PCR set-up
- Accurate - proofreading component delivers 3.5x higher fidelity than Taq DNA Polymerase, enabling cloning of PCR products
Product Description
MyFi™ has been developed to give reliable amplification of targets up to 10 kb from challenging and complex targets, even in the presence of PCR inhibitors. MyFi is therefore ideal for amplification of cDNA libraries, complex genomic fragments and GC-rich targets. Additionally, MyFi shows improved tolerance to PCR inhibitors, thereby enabling reliable detection from samples from which DNA is difficult to purify. Furthermore, its unique buffering system and enzyme blend promote highly sensitive amplification of even very low-copy number targets. The proofreading ability of MyFi allows all PCR products to be cloned. The inclusion of MyTaq HS means MyFi generates PCR products with 3’-A overhangs, which is perfect for TA cloning. MyFi has the added convenience of room temperature reaction assembly, to avoid non-specific amplification and primer-dimer formation.
MyFi Mix is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. The inclusion of dNTPs, MgCl2 and enhancers at optimal concentrations, helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.
Applications
- Amplification of challenging and complex templates
- Inhibitor-tolerant PCR
- Robust PCR
- TA cloning
PCR Enzyme Guide
Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your researchPCR Selection Chart
Select the best reagent for your researchMyFi Mix is more sensitive than our current Taq and so easy to use/set up. Nice product.
Aberystwyth University, IBERS, UK
Product Selection
Please refer to the PCR Selection Chart to confirm the recommended product for your PCR application.Resources
Documents
Certification of Analysis (COAs)
Specification
Components
|
Reagent |
100 Reactions |
500 Reactions |
|
MyFi Mix, 2x |
2 x 1.25 mL |
10 x 1.25 mL |
Volume
- BIO-25049: 100 x 50 µL Reactions: 2 x 1.25 mL
- BIO-25050: 500 x 50 µL Reactions: 10 x 1.25 mL
Concentration
- 2x
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date indicated on the outer box label.
Shipping conditions
Shipped on Dry Ice or Blue Ice.
FAQs
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
| Observation | Recommended Solution(s) |
| No or low PCR yield | Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments. |
| Primers degraded – check quality and age of the primers. | |
| Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM. | |
| Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration. | |
| Template concentration too low – Increase concentration of template. | |
| Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated. | |
| Multiple Bands | Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers. |
| Prepare master mixes on ice or use a heat-activated polymerase. | |
| For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity. | |
| Smearing or artifacts | Template concentration too high. Prepare serial dilutions of template. |
| Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands. | |
| Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments. | |
| Extension time too long. Reduce extension time in 0.5-1 minute increments. |
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